| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,429,321 | T→C | 39.2% | M88V (ATG→GTG) | wzzE ← | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,429,321 | 0 | T | C | 39.2% | 56.5 / 90.2 | 79 | M88V (ATG→GTG) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base T (25/23); new base C (19/12); total (44/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
GCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGTT > minE/2429257‑2429391 | gCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTg < 1:547316/70‑1 (MQ=255) cAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAAc < 1:2622734/71‑1 (MQ=255) cAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAAc < 1:2447942/71‑1 (MQ=255) aGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACg < 1:924303/71‑1 (MQ=255) aGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACg < 1:185071/71‑1 (MQ=255) ttGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAAc < 1:2895678/68‑1 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:2504740/1‑68 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3315960/1‑68 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3500917/1‑68 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3525196/1‑68 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:540473/1‑68 (MQ=255) gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa > 1:1263212/1‑71 (MQ=255) aTAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATc < 1:714988/71‑1 (MQ=255) tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATcc < 1:3246396/71‑1 (MQ=255) tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATc > 1:31935/1‑70 (MQ=255) tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATcc < 1:3119291/71‑1 (MQ=255) tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATcc < 1:2455715/71‑1 (MQ=255) aCAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACg > 1:1346540/1‑62 (MQ=255) cAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCAt > 1:1156060/1‑52 (MQ=255) cAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAgg < 1:1849367/71‑1 (MQ=255) aaaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:1696006/1‑71 (MQ=255) aaaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa > 1:3593754/1‑64 (MQ=255) aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:3556421/1‑70 (MQ=255) aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:644498/1‑70 (MQ=255) aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTa < 1:3196334/71‑1 (MQ=255) aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTa > 1:1944787/1‑71 (MQ=255) aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:1759875/1‑71 (MQ=255) ctctTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTAc < 1:3303564/71‑1 (MQ=255) ctctTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTAc > 1:834540/1‑71 (MQ=255) tttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGc > 1:2010807/1‑70 (MQ=255) ttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCa > 1:1551769/1‑70 (MQ=255) tataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa > 1:3559689/1‑70 (MQ=255) ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGtt > 1:1630024/1‑45 (MQ=255) ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGtt > 1:1584301/1‑45 (MQ=255) ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaa > 1:704229/1‑70 (MQ=255) taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:1812497/1‑71 (MQ=255) taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:708006/1‑71 (MQ=255) taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaaa < 1:2379172/70‑1 (MQ=255) ggCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAAt < 1:3306147/70‑1 (MQ=255) gCTTCGTCCATGACC‑ATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:3628642/71‑1 (MQ=255) cTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:2915930/71‑1 (MQ=255) cTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:2384419/71‑1 (MQ=255) ttCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgct > 1:3555059/1‑71 (MQ=255) ggccATGACCGATGGGTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:3533881/69‑1 (MQ=255) gTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:644628/71‑1 (MQ=255) gTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:1325029/71‑1 (MQ=255) ccATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:2673022/1‑53 (MQ=255) ccATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:2687893/1‑53 (MQ=255) cATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgctgc > 1:3116106/1‑70 (MQ=255) cATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCg < 1:2207106/71‑1 (MQ=255) aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:2757347/1‑54 (MQ=255) aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:3713097/1‑54 (MQ=255) aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:2131458/1‑54 (MQ=255) tGACCGATGGTTGATCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa > 1:2770613/1‑55 (MQ=255) gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1499991/1‑70 (MQ=255) gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1880210/1‑70 (MQ=255) gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1747700/1‑70 (MQ=255) aCCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGt < 1:567342/70‑1 (MQ=255) aCCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGt < 1:3116165/70‑1 (MQ=255) aCCGATGGTTGGACGGCAGAAGCCATGTTTGAACgg < 1:583762/36‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:354504/42‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2365807/42‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:339099/42‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:3649841/42‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2757902/42‑1 (MQ=255) gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2105912/42‑1 (MQ=255) gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTa < 1:562441/60‑1 (MQ=255) gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc > 1:3438588/1‑69 (MQ=255) gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc > 1:2553210/1‑69 (MQ=255) ggCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCa > 1:3049481/1‑71 (MQ=255) gCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCa > 1:14983/1‑70 (MQ=255) cAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata < 1:1961737/71‑1 (MQ=255) cAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata > 1:972938/1‑71 (MQ=255) gAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGa < 1:3232260/50‑1 (MQ=255) aaGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCAGCGAGTAATATCCCCCCAGCATATTc < 1:647217/71‑1 (MQ=255) gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:2122894/1‑35 (MQ=255) gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:2377163/1‑35 (MQ=255) gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:3249031/1‑35 (MQ=255) aTGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGt > 1:2420187/1‑71 (MQ=255) aTGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGt > 1:2457498/1‑71 (MQ=255) tGTTTGAAGGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGtt > 1:3131841/1‑71 (MQ=255) | GCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGTT > minE/2429257‑2429391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |