Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,460,541 T→G 10.0% intergenic (+159/‑220) mioC → / → gidA FMN‑binding protein MioC/glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,460,5410TG10.0% 218.7 / 20.5 90intergenic (+159/‑220)mioC/gidAFMN‑binding protein MioC/glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base T (29/52);  new base G (3/6);  total (32/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TTTGAGTTGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATCCTA  >  minE/2460472‑2460609
                                                                     |                                                                    
tttGAGTTGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGtt                                                                      >  1:171261/1‑70 (MQ=255)
tttGAGTTGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGtt                                                                      <  1:2105709/70‑1 (MQ=255)
      ttGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTAACTGTGAATGATc                                                               <  1:3549979/71‑1 (MQ=39)
      ttGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTAACTGTGAATGATc                                                               <  1:367646/71‑1 (MQ=39)
        gtgtATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCt                                                             >  1:377912/1‑71 (MQ=255)
         tgtATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCt                                                             <  1:2137029/70‑1 (MQ=255)
           tataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          <  1:677538/71‑1 (MQ=255)
           tataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          <  1:2407355/71‑1 (MQ=255)
           tataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          <  1:3387845/71‑1 (MQ=255)
           tataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          <  1:1860000/71‑1 (MQ=255)
            ataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          >  1:2405665/1‑70 (MQ=255)
            ataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          >  1:3409398/1‑70 (MQ=255)
            ataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          >  1:2478203/1‑70 (MQ=255)
            ataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          >  1:2874339/1‑70 (MQ=255)
            ataACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGATAATGATCCTTTc                                                          >  1:2540650/1‑70 (MQ=255)
             taACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTc                                                          >  1:495852/1‑69 (MQ=255)
              aaCCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGAt                                                                <  1:2124301/62‑1 (MQ=255)
               aCCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAgg                                                      <  1:620297/71‑1 (MQ=255)
               aCCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAgg                                                      <  1:983127/71‑1 (MQ=255)
               aCCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAg                                                       <  1:703904/70‑1 (MQ=255)
                 cccTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGgtt                                                    <  1:2514603/71‑1 (MQ=255)
                  ccTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTcc                                                         <  1:337209/65‑1 (MQ=255)
                  ccTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTcc                                                         <  1:1663440/65‑1 (MQ=255)
                  ccTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttg                                                   <  1:1163588/71‑1 (MQ=255)
                   cTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttgt                                                  >  1:1717186/1‑71 (MQ=255)
                   cTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttgt                                                  >  1:245900/1‑71 (MQ=255)
                      aTTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGa                                               >  1:749467/1‑71 (MQ=255)
                         cTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATc                                             <  1:2912112/70‑1 (MQ=255)
                            aTCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaa                                         <  1:2715870/71‑1 (MQ=255)
                            aTCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaa                                         <  1:3558506/71‑1 (MQ=255)
                               ccAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTa                                                                     <  1:2880580/40‑1 (MQ=255)
                               ccAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTa                                                                     <  1:152805/40‑1 (MQ=255)
                               ccAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaaaa                                       <  1:992486/70‑1 (MQ=255)
                               ccAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAg                                      <  1:3044816/71‑1 (MQ=255)
                                  gCTTATACGGTCCAGGATCACCGATCATTCACAGTTa                                                                     >  1:786773/1‑37 (MQ=255)
                                  gCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCg                                   >  1:727487/1‑71 (MQ=255)
                                   cTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCg                                   >  1:2316318/1‑70 (MQ=255)
                                      ataCGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGa                                 <  1:2748717/69‑1 (MQ=255)
                                      ataCGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGa                                 <  1:1865724/69‑1 (MQ=255)
                                       taCGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGAta                               <  1:2450134/70‑2 (MQ=255)
                                        aCGGTCCAGGATCACCGATCATTCACAGTTTATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATctt                             >  1:2881554/1‑69 (MQ=255)
                                        aCGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGa                                 <  1:2008965/67‑1 (MQ=255)
                                        aCGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATctt                             >  1:3250974/1‑69 (MQ=255)
                                         cGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:992122/70‑1 (MQ=255)
                                         cGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:705751/70‑1 (MQ=255)
                                         cGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:3062997/70‑1 (MQ=255)
                                            tCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGtt                         <  1:1486331/71‑1 (MQ=255)
                                              cAGGATCACCGATCATTCAGAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGa                                 <  1:1453862/61‑1 (MQ=255)
                                              cAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGtt                         <  1:1040926/69‑1 (MQ=255)
                                              cAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTa                        <  1:278424/70‑1 (MQ=255)
                                                ggATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATc                      >  1:2435129/1‑70 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttgt                                                  <  1:619702/41‑1 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttgt                                                  <  1:3179785/41‑1 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGgttgt                                                  <  1:2952612/41‑1 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGcc                                    <  1:2730616/55‑1 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCca                    >  1:552584/1‑71 (MQ=255)
                                                 gATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCca                    >  1:2693514/1‑71 (MQ=255)
                                                  aTCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCcac                   <  1:2205723/71‑1 (MQ=255)
                                                  aTCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCcac                   <  1:3441014/71‑1 (MQ=255)
                                                  aTCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCca                    >  1:3107661/1‑70 (MQ=255)
                                                   tCACCGATCATTCACAGTTAATGATCCTTTCCAGGttgttg                                                >  1:3551329/1‑41 (MQ=255)
                                                     tccGATCATTCACAGTTAATGATCCCTTCCAGgttgt                                                  <  1:2694646/36‑1 (MQ=255)
                                                     aCCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaa                                         <  1:3218198/46‑1 (MQ=255)
                                                     aCCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaa                                         <  1:1188125/46‑1 (MQ=255)
                                                     aCCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTaa                                         <  1:2784009/46‑1 (MQ=255)
                                                        gATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCtt                                           <  1:3492452/41‑1 (MQ=255)
                                                        gATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAggg               >  1:2087854/1‑69 (MQ=255)
                                                         aTCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCtt                                           <  1:505806/40‑1 (MQ=255)
                                                           cATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTg          >  1:9120/1‑71 (MQ=255)
                                                           cATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTg          >  1:2186796/1‑71 (MQ=255)
                                                           cATTAACTGTGAATGATCCTTTCCAGGTTGTTGATCt                                            >  1:707858/1‑37 (MQ=21)
                                                           cATTAACTGTGAATGATCCTTTCCAGGTTGTTGATCt                                            >  1:1785338/1‑37 (MQ=21)
                                                           cATTAACTGTGAATGATCCTTTCCAGGTTGTTGATCt                                            >  1:1293796/1‑37 (MQ=21)
                                                           cATTAACTGTGAATGATCCTTTCCAGGTTGTTGATCTTAAAAg                                      <  1:3617489/43‑1 (MQ=25)
                                                            aTTAACTGTGAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGTATCCTTGTTATCCACAGGGCAGTg          <  1:464127/70‑1 (MQ=255)
                                                            aTTAACTGTGAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTg          <  1:3403779/70‑1 (MQ=255)
                                                            aTTAACTGTGAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTg          <  1:3706567/70‑1 (MQ=255)
                                                             ttCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCg        <  1:2104211/71‑1 (MQ=255)
                                                              tCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:3563039/49‑1 (MQ=255)
                                                              tCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:2295619/49‑1 (MQ=255)
                                                              tCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCt                             <  1:1847744/49‑1 (MQ=255)
                                                               cacaGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGa       <  1:2221108/70‑1 (MQ=255)
                                                                acaGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTg                           >  1:3640017/1‑49 (MQ=255)
                                                                acaGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGa       <  1:1529369/69‑1 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTTTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATcc    >  1:2113949/1‑70 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTTTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATcc    >  1:2109616/1‑70 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATcc                              <  1:3318294/44‑1 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATc     >  1:1262687/1‑69 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATc     >  1:1668278/1‑69 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATCCt   <  1:994412/71‑1 (MQ=255)
                                                                  aGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATACACAGGGCAGTGCGATc     >  1:173599/1‑69 (MQ=255)
                                                                   gTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATCCta  <  1:2975756/71‑1 (MQ=255)
                                                                     |                                                                    
TTTGAGTTGTGTATAACCCCTCATTCTGATCCCAGCTTATACGGTCCAGGATCACCGATCATTCACAGTTAATGATCCTTTCCAGGTTGTTGATCTTAAAAGCCGGATCCTTGTTATCCACAGGGCAGTGCGATCCTA  >  minE/2460472‑2460609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: