| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,537,451 | C→A | 7.9% | F48L (TTC→TTA) | rfaB → | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,537,451 | 0 | C | A | 7.9% | 332.9 / 8.5 | 100 | F48L (TTC→TTA) | rfaB | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (37/55); new base A (5/3); total (42/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCATGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCA > minE/2537381‑2537518 | gcATGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTc > 1:2318461/1‑71 (MQ=255)gcATGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTc > 1:515550/1‑71 (MQ=255) aTGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtt < 1:3003224/71‑1 (MQ=255) aTGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtt < 1:3440847/71‑1 (MQ=255) tttaaCTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtttt < 1:1841914/68‑1 (MQ=255) ggAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtttt > 1:2789135/1‑71 (MQ=255) ggAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtttt > 1:2301995/1‑71 (MQ=255) ggAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCtttt > 1:736109/1‑71 (MQ=255) ggAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCttt > 1:3572625/1‑70 (MQ=255) gAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTg > 1:1538373/1‑71 (MQ=255) gAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTg < 1:725397/71‑1 (MQ=255) gAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATAT‑CAAAGTGAAATGTTTTTCTTTTGt > 1:1002075/1‑71 (MQ=255) aaCTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTc > 1:2559149/1‑71 (MQ=255) cTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:2991530/71‑1 (MQ=255) tCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTaa < 1:634715/70‑1 (MQ=255) ccGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGCGAAATGTTTTTCTTTTGTCGTAATGAc > 1:483947/1‑70 (MQ=255) cGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACa < 1:309779/70‑1 (MQ=255) gTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCttt < 1:794850/56‑1 (MQ=255) gTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:1466531/71‑1 (MQ=255) gTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaa > 1:3388238/1‑70 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:3653904/71‑1 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:2036471/71‑1 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:481659/71‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACa < 1:54414/66‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaaga < 1:3635936/71‑3 (MQ=37) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaag < 1:2173074/70‑2 (MQ=38) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATgg < 1:2073887/71‑1 (MQ=255) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATg > 1:2284599/1‑70 (MQ=255) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaaga < 1:534954/70‑3 (MQ=37) gTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTc < 1:2574410/48‑1 (MQ=255) gTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTc < 1:366643/48‑1 (MQ=255) ttattaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaa < 1:1647480/64‑1 (MQ=255) tattaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:120517/1‑70 (MQ=255) attaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:867648/1‑69 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:487334/70‑1 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2038378/70‑1 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAAGGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2491377/70‑1 (MQ=255) actacCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:2914086/70‑1 (MQ=255) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATg > 1:16510/1‑71 (MQ=255) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:3561184/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:3736275/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:4607/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:2668984/1‑55 (MQ=37) acCTTCAGGCAACAACAAATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:1639817/1‑55 (MQ=37) ccTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATgg > 1:3026839/1‑71 (MQ=255) cTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:3165351/46‑1 (MQ=255) ttCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:807924/54‑1 (MQ=255) ttCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:65004/54‑1 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa > 1:1332982/1‑54 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATgg < 1:264045/68‑1 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg > 1:2135480/1‑71 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCt < 1:2417253/69‑1 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg < 1:2135384/70‑1 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg < 1:590601/70‑1 (MQ=255) aGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTgg < 1:1757676/70‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTATTGACAAAATGGATAAGGGATGGCTGGaa < 1:2700041/71‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATTGATAAGGGATGGCTgg > 1:1771285/1‑69 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGc > 1:3002319/1‑66 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTgg > 1:3647621/1‑69 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGaa < 1:1808028/71‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGACTgg > 1:2066720/1‑69 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:1044545/59‑1 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:1742374/59‑1 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:3408371/59‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:742643/38‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:1984969/38‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:3369893/38‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGt < 1:3099492/38‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg > 1:2153852/1‑70 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg > 1:265366/1‑70 (MQ=255) caacaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTaa > 1:2997070/1‑38 (MQ=255) caacaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTAt > 1:158037/1‑71 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCt < 1:2808014/60‑1 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTAtt > 1:3297200/1‑70 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTa < 1:2555318/71‑1 (MQ=255) atatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTaaa > 1:1704257/1‑71 (MQ=255) tatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTa > 1:3015526/1‑65 (MQ=255) tatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTa > 1:201377/1‑65 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:3154336/46‑1 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:1851681/46‑1 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2037448/46‑1 (MQ=255) ccAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:2044104/1‑43 (MQ=255) cAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTc < 1:3585518/71‑1 (MQ=255) aaGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTc > 1:1582783/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATa > 1:533305/1‑40 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATa > 1:2381959/1‑40 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCa > 1:2071015/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCa > 1:3483536/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:3559369/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:857051/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:1690662/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:1407522/71‑1 (MQ=255) aaaTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg < 1:2142874/52‑1 (MQ=255) aaTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAt < 1:1951773/71‑1 (MQ=255) aTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAtt < 1:1633212/71‑1 (MQ=255) aTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAtt < 1:762266/71‑1 (MQ=255) tGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAttt < 1:879749/71‑1 (MQ=255) tttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTc > 1:100022/1‑69 (MQ=255) tttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTc > 1:3646962/1‑69 (MQ=255) ttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCa < 1:1691131/70‑1 (MQ=255) | GCATGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCA > minE/2537381‑2537518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |