| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,537,461 | A→T | 9.8% | N52Y (AAT→TAT) ‡ | rfaB → | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,537,461 | 0 | A | T | 9.8% | 262.2 / 14.9 | 91 | N52Y (AAT→TAT) ‡ | rfaB | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (33/49); new base T (6/3); total (39/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGTTTAG > minE/2537393‑2537530 | tCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTaa < 1:634715/70‑1 (MQ=255) ccGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGCGAAATGTTTTTCTTTTGTCGTAATGAc > 1:483947/1‑70 (MQ=255) cGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACa < 1:309779/70‑1 (MQ=255) gTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:1466531/71‑1 (MQ=255) gTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaa > 1:3388238/1‑70 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:481659/71‑1 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:3653904/71‑1 (MQ=255) tGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa < 1:2036471/71‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACa < 1:1800207/66‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACa < 1:54414/66‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAAt < 1:40267/70‑1 (MQ=255) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaaga < 1:3635936/71‑3 (MQ=37) aTGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaag < 1:2173074/70‑2 (MQ=38) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATgg < 1:2073887/71‑1 (MQ=255) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATg > 1:2284599/1‑70 (MQ=255) tGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACaaaaga < 1:534954/70‑3 (MQ=37) ttattaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaa < 1:1647480/64‑1 (MQ=255) tattaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:120517/1‑70 (MQ=255) attaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:867648/1‑69 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2038378/70‑1 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:487334/70‑1 (MQ=255) ttaCTACCTTCAGGCAACAACATATCCAAAGTGAAAGGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2491377/70‑1 (MQ=255) actacCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:2914086/70‑1 (MQ=255) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATg > 1:16510/1‑71 (MQ=255) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:3736275/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:3561184/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:4607/1‑55 (MQ=37) acCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:2668984/1‑55 (MQ=37) acCTTCAGGCAACAACAAATCCAAAGTGAAATGTTTTTATTTTGTCATTACGACa > 1:1639817/1‑55 (MQ=37) ccTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATgg > 1:3026839/1‑71 (MQ=255) cTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTa < 1:304357/47‑1 (MQ=255) ttCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:65004/54‑1 (MQ=255) ttCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaa < 1:807924/54‑1 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACaaaa > 1:1332982/1‑54 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATgg < 1:264045/68‑1 (MQ=255) tCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg > 1:2135480/1‑71 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCt < 1:2417253/69‑1 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg < 1:590601/70‑1 (MQ=255) cAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTg < 1:2135384/70‑1 (MQ=255) aGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTgg < 1:1757676/70‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTATTGACAAAATGGATAAGGGATGGCTGGaa < 1:2700041/71‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATTGATAAGGGATGGCTgg > 1:1771285/1‑69 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGc > 1:3002319/1‑66 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTgg > 1:3647621/1‑69 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGaa < 1:1808028/71‑1 (MQ=255) ggCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGACTgg > 1:2066720/1‑69 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:1044545/59‑1 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:3408371/59‑1 (MQ=255) gCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAgg < 1:1742374/59‑1 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg > 1:2153852/1‑70 (MQ=255) aacaacATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg > 1:265366/1‑70 (MQ=255) caacaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTaa > 1:2997070/1‑38 (MQ=255) caacaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTAt > 1:158037/1‑71 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCt < 1:2808014/60‑1 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTAtt > 1:3297200/1‑70 (MQ=255) acaTATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTa < 1:2555318/71‑1 (MQ=255) atatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTaaa > 1:1704257/1‑71 (MQ=255) tatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTa > 1:3015526/1‑65 (MQ=255) tatCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTa > 1:201377/1‑65 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:1851681/46‑1 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:3154336/46‑1 (MQ=255) tCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATaa < 1:2037448/46‑1 (MQ=255) ccAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGAt > 1:2044104/1‑43 (MQ=255) cAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTc < 1:3585518/71‑1 (MQ=255) aaGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTc > 1:1582783/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATa > 1:2381959/1‑40 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATa > 1:533305/1‑40 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCa > 1:2071015/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCa > 1:3483536/1‑69 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:3559369/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:1407522/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:857051/71‑1 (MQ=255) aGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATg < 1:1690662/71‑1 (MQ=255) aaaTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAgg < 1:2142874/52‑1 (MQ=255) aaTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAt < 1:1951773/71‑1 (MQ=255) aTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAtt < 1:762266/71‑1 (MQ=255) aTGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAtt < 1:1633212/71‑1 (MQ=255) tGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAttt < 1:879749/71‑1 (MQ=255) tttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTc > 1:3646962/1‑69 (MQ=255) tttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTc > 1:100022/1‑69 (MQ=255) ttCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCa < 1:1691131/70‑1 (MQ=255) tttGTCATTACGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATAtt > 1:563514/1‑71 (MQ=255) tGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTc < 1:2937104/50‑1 (MQ=255) tGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCg > 1:1524973/1‑71 (MQ=255) tGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCg > 1:614798/1‑71 (MQ=255) tGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCg > 1:138567/1‑71 (MQ=255) tGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCg > 1:2847328/1‑71 (MQ=255) tCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGtt < 1:496993/71‑1 (MQ=255) tCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGtt < 1:3296507/71‑1 (MQ=255) tCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGt < 1:718542/70‑1 (MQ=255) gTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGttt < 1:1410971/70‑1 (MQ=255) gTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGttt < 1:2837279/70‑1 (MQ=255) tAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCAtt < 1:1604015/54‑1 (MQ=255) tAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGTTTAg > 1:2996814/1‑71 (MQ=255) | TCATCCGTGATGTTATTACTACCTTCAGGCAACAACATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTGGAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGTTTAG > minE/2537393‑2537530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |