| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,174 | G→C | 5.4% | P368R (CCG→CGG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,174 | 0 | G | C | 5.4% | 240.4 / 2.9 | 74 | P368R (CCG→CGG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (35/35); new base C (2/2); total (37/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
TTGATGGTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAAC > minE/2586105‑2586244 | ttGATGGTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCgg < 1:2343186/71‑1 (MQ=255)ttGATGGTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCgg > 1:1471265/1‑71 (MQ=255) tGGTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGgatg < 1:2256472/71‑1 (MQ=255) gTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGgatgtt < 1:1284896/71‑3 (MQ=255) gAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg > 1:3318135/1‑71 (MQ=255) aaaCCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg < 1:3101951/70‑1 (MQ=255) aaCCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCgg > 1:3704587/1‑61 (MQ=255) aaCCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCgg > 1:1687016/1‑61 (MQ=255) accgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTgg < 1:581848/71‑1 (MQ=255) accgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTgg < 1:2912076/71‑1 (MQ=255) accgacagCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTgg > 1:1641868/1‑71 (MQ=255) ccgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg < 1:105942/67‑1 (MQ=255) ccgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg < 1:2715280/67‑1 (MQ=255) ccgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg < 1:1515990/67‑1 (MQ=255) ccgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATtg < 1:1243369/67‑1 (MQ=255) ccgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGc < 1:292813/71‑1 (MQ=255) cgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCa > 1:327009/1‑71 (MQ=255) cgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCa > 1:19074/1‑71 (MQ=255) cgaccgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCa > 1:331483/1‑71 (MQ=255) accgCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACg < 1:2218634/71‑1 (MQ=255) cgccgACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGa < 1:1128298/71‑1 (MQ=255) ccgACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGaaa < 1:2914440/71‑1 (MQ=255) ccgACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGaaa < 1:2910231/71‑1 (MQ=255) ccgACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGaaa > 1:2427262/1‑71 (MQ=255) gACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAAtt < 1:3677517/71‑1 (MQ=255) gACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAAtt < 1:3225248/71‑1 (MQ=255) aCAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc > 1:2734120/1‑71 (MQ=255) aCAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc > 1:2592374/1‑71 (MQ=255) aCAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:949799/71‑1 (MQ=255) cAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:2786680/70‑1 (MQ=255) aaGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTg > 1:975482/1‑71 (MQ=255) aaGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTg > 1:3164775/1‑71 (MQ=255) gATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGc > 1:3246673/1‑70 (MQ=255) tCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa < 1:2627765/71‑1 (MQ=255) tCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaa < 1:470624/70‑1 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:2340945/1‑70 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:3196857/1‑70 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:1933909/1‑70 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:1490418/1‑70 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:645919/1‑70 (MQ=255) cGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaa > 1:3433945/1‑70 (MQ=255) cGCAAAAGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc > 1:476352/1‑64 (MQ=255) gCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGTCACGGAAATTCTGCaaaaa < 1:887505/71‑1 (MQ=255) gCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaa < 1:1956740/70‑1 (MQ=255) cAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:2202582/1‑70 (MQ=255) aaaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:2755802/1‑69 (MQ=255) aaaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:1479832/1‑69 (MQ=255) aaaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:2612917/1‑69 (MQ=255) aaaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:1830737/1‑69 (MQ=255) aaaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:2989303/1‑69 (MQ=255) aaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:855571/1‑68 (MQ=255) aaCGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAg < 1:3239687/71‑1 (MQ=255) cGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGAt < 1:1361341/71‑1 (MQ=255) cGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATg < 1:3148438/71‑1 (MQ=255) cGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATg < 1:3208608/71‑1 (MQ=255) gaATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGAc > 1:2973894/1‑70 (MQ=255) gaATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGAc > 1:2884051/1‑70 (MQ=255) aaTAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCaaaaa > 1:307445/1‑57 (MQ=255) aaCCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAgc > 1:1516235/1‑69 (MQ=255) accaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGaa < 1:1832919/71‑1 (MQ=255) accaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGaa < 1:3106758/71‑1 (MQ=255) accaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGaa < 1:908787/71‑1 (MQ=255) accaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGaa < 1:3143398/71‑1 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:887869/44‑1 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:1514033/44‑1 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:3178140/44‑1 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTc < 1:287674/44‑1 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAgg > 1:3509186/1‑68 (MQ=255) ccaccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAgg > 1:913836/1‑68 (MQ=255) ccGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGAc > 1:1235509/1‑70 (MQ=255) cGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGAc > 1:2067913/1‑69 (MQ=255) ggTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAg < 1:2921220/71‑1 (MQ=255) gTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAg > 1:972558/1‑70 (MQ=255) gTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAg > 1:413418/1‑70 (MQ=255) cacAATCATCCCGACGATTGTGGCACGGAAATTCTGCAAAAACAGATACAt < 1:813345/49‑1 (MQ=21) cacAATCATCCCGACGATTGTGGCACGGAAATTCTGCAAAAACAGATACAt < 1:3489275/49‑1 (MQ=21) cAATCATCCCGACGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGa > 1:2843095/1‑51 (MQ=25) cAATCATCCCGACGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGa > 1:3098548/1‑51 (MQ=25) tcgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCa < 1:1750214/51‑1 (MQ=255) tcgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGcc < 1:939492/71‑1 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATTACCAGGAAGACTAGGATGATAGCCt > 1:68112/1‑71 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGcc < 1:968366/70‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTTCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCt > 1:1127860/1‑70 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAAc > 1:450100/1‑36 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAg > 1:1039440/1‑50 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAg > 1:783366/1‑50 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAg > 1:1096080/1‑50 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAg > 1:2784802/1‑50 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAg > 1:1362154/1‑50 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgatg < 1:170488/63‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgatg < 1:2165725/63‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgatg < 1:2489156/63‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgatg < 1:647807/63‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgatg < 1:2828473/63‑1 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCt > 1:2387138/1‑70 (MQ=255) gggATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc < 1:2736986/65‑1 (MQ=255) gggATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAAc > 1:2055405/1‑71 (MQ=255) gggATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAAc > 1:2041426/1‑71 (MQ=255) gggATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAAc < 1:311468/71‑1 (MQ=255) | TTGATGGTGAAACCGACCGCCGACAAGATCGCAAACGTCCCGAGAATAACCACCGGTACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAAC > minE/2586105‑2586244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |