| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,590,091 | A→T | 15.3% | intergenic (‑767/+32) | gadE ← / ← hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,590,091 | 0 | A | T | 15.3% | 135.3 / 14.5 | 52 | intergenic (‑767/+32) | gadE/hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (19/25); new base T (4/4); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
AATGATAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCTGAA > minE/2590021‑2590158 | aaTGATAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCaa < 1:1774916/71‑1 (MQ=255) tGATAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGt < 1:3697609/71‑1 (MQ=255) tGATAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGt > 1:1508633/1‑71 (MQ=255) tAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGAc < 1:551641/71‑1 (MQ=38) aGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGAc > 1:958681/1‑70 (MQ=255) tGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTAt > 1:1770637/1‑71 (MQ=255) cgcAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATg < 1:1733957/70‑1 (MQ=255) cgcAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGa > 1:2865140/1‑71 (MQ=255) taaTTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATc < 1:3554410/70‑1 (MQ=255) cAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATc < 1:1289165/71‑1 (MQ=255) aaTTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATcc > 1:2736671/1‑71 (MQ=255) aaTTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATcc > 1:2387378/1‑71 (MQ=255) aaTTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATcc < 1:1887424/71‑1 (MQ=255) tGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTAt < 1:1348074/63‑1 (MQ=25) tGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTAt < 1:413759/63‑1 (MQ=25) gATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCggg < 1:1135893/70‑1 (MQ=255) aTCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTATGAt > 1:174023/1‑64 (MQ=25) aTCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTATGAt > 1:1482704/1‑64 (MQ=25) aTCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGt < 1:2937992/70‑1 (MQ=255) caacaCTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACa < 1:2441114/69‑1 (MQ=255) aacaCTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAAcc > 1:3008340/1‑71 (MQ=255) aacaCTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAAcc > 1:635911/1‑71 (MQ=255) cacTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACt < 1:1598542/51‑1 (MQ=21) cacTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCg > 1:1481494/1‑70 (MQ=255) acTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGt < 1:2813525/46‑1 (MQ=255) cTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCgggg > 1:1820606/1‑71 (MQ=255) gCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCgg < 1:360923/56‑1 (MQ=255) cGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTATGa > 1:2501433/1‑50 (MQ=18) cGTAGCGGAGAGAGTATTAATCGGATCATAGTCACTTGATGTGACTATGa > 1:1929548/1‑50 (MQ=18) gTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAat < 1:352939/71‑1 (MQ=255) tAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAatt < 1:80342/71‑1 (MQ=255) gCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAattat < 1:2055885/71‑1 (MQ=255) gCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGAGACTATGATCCGGGTGACAACCGGGGTAattat > 1:1306696/1‑71 (MQ=255) gagagaGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATtgc < 1:3732275/71‑1 (MQ=255) gagagTATTAATCGGATCATAGTCACATCAAGTGACTa > 1:3349736/1‑38 (MQ=255) agagTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATtgctgc > 1:1446948/1‑71 (MQ=255) gTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTa < 1:2124720/71‑1 (MQ=255) tATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTaa > 1:2770297/1‑71 (MQ=255) aaTCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGa > 1:1868235/1‑70 (MQ=255) aTCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGaa > 1:3273105/1‑70 (MQ=255) ggATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACaaa < 1:1455562/71‑1 (MQ=255) gATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAAc < 1:1171747/71‑1 (MQ=255) gATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAAc < 1:2632683/71‑1 (MQ=255) cATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCtt < 1:3148782/57‑1 (MQ=255) aGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCg > 1:930581/1‑70 (MQ=255) gTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCg > 1:2874615/1‑69 (MQ=255) acaTCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGc < 1:3252652/70‑1 (MQ=255) acaTCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGc < 1:597434/70‑1 (MQ=255) acaTCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCt < 1:2859995/71‑1 (MQ=255) acaTCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCt < 1:2065624/71‑1 (MQ=255) caTCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCTg < 1:3632356/71‑1 (MQ=255) tCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCTGaa < 1:2699035/71‑1 (MQ=255) cAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTg > 1:1890504/1‑59 (MQ=255) aaGTGACTATGATCCGGGTGACAACCGGGGTAatt < 1:558175/35‑1 (MQ=255) | AATGATAGTGCGCAATTGATCTACAACACTGCGTAGCGGAGAGAGTATTAATCGGATCATAGTCACATCAAGTGACTATGATCCGGGTGACAACCGGGGTAATTATTGCTGCTTAACGAACAAACTGGCGAAGCTGAA > minE/2590021‑2590158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |