| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,687,465 | C→T | 13.6% | G272S (GGC→AGC) | pck ← | phosphoenolpyruvate carboxykinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,687,465 | 0 | C | T | 13.6% | 216.2 / 22.4 | 81 | G272S (GGC→AGC) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base C (35/35); new base T (4/7); total (39/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
CTTTCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCGGAA > minE/2687396‑2687532 | cttTCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCg > 1:2691364/1‑71 (MQ=255) tttCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCgt > 1:1723192/1‑71 (MQ=255) tCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCgt < 1:3028060/69‑1 (MQ=255) tCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCgt < 1:2780853/69‑1 (MQ=255) cGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCgtgt < 1:2919532/70‑1 (MQ=255) cGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCgtgt < 1:1640155/70‑1 (MQ=255) gATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGc > 1:21455/1‑71 (MQ=255) tAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGcc > 1:1679309/1‑70 (MQ=255) aGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTc < 1:2014841/63‑1 (MQ=255) gTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCaa < 1:3270685/70‑1 (MQ=255) gTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCaa > 1:2881381/1‑70 (MQ=255) gTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCaa < 1:2442238/70‑1 (MQ=255) gTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAAt > 1:2591020/1‑71 (MQ=255) gTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAAt > 1:2415001/1‑71 (MQ=255) ttttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAAt > 1:2109869/1‑69 (MQ=255) ttttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAAt > 1:1149818/1‑69 (MQ=255) tttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAg > 1:2347892/1‑71 (MQ=255) tttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATc > 1:3014116/1‑69 (MQ=255) tttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCa < 1:3044503/70‑1 (MQ=255) ttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAg < 1:2850975/70‑1 (MQ=255) ttGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAg < 1:1918582/70‑1 (MQ=255) tGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAgg < 1:884911/70‑1 (MQ=255) tGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAgg < 1:587323/70‑1 (MQ=255) gCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGc > 1:1384732/1‑70 (MQ=255) gCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGc > 1:1638629/1‑70 (MQ=255) tAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACg < 1:2709781/71‑1 (MQ=255) cagcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGttt < 1:1239351/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACGTTTc < 1:186694/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACGTTTc < 1:2914411/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACGTTTc < 1:1370226/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACGTTTc < 1:1425809/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTc < 1:2459443/71‑1 (MQ=255) agcCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTc > 1:3506998/1‑71 (MQ=255) gccTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGt < 1:2379382/71‑1 (MQ=255) ccTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGt > 1:3590692/1‑70 (MQ=255) tCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAg < 1:2437317/53‑1 (MQ=255) cgtaGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCgg < 1:3425881/66‑1 (MQ=255) gAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCAt > 1:3320562/1‑41 (MQ=255) gAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTc > 1:831352/1‑61 (MQ=255) gAAGTTAAACACGCCGTCAACGTCCCAGCCGTGTTCg > 1:2196263/1‑37 (MQ=255) aaGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGa < 1:1752270/71‑1 (MQ=255) aGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGa > 1:370413/1‑70 (MQ=255) aGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGa > 1:3478096/1‑70 (MQ=255) ttAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCg < 1:2992053/63‑1 (MQ=255) tAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGa > 1:1909311/1‑67 (MQ=255) tAAACACGCCGTCATCGTCCCAGCCGTCTTCGTCATCGACAATCAGGCGACGTTTCg > 1:381782/1‑57 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGt > 1:2652128/1‑52 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGt > 1:111201/1‑52 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGt > 1:299563/1‑52 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCgg > 1:1699535/1‑57 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCgg > 1:3649007/1‑57 (MQ=255) aaaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAgg < 1:3367215/70‑1 (MQ=255) aaCACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAggg < 1:2011409/70‑1 (MQ=255) cacGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGggtgg > 1:3028556/1‑71 (MQ=255) cacGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGggtgg < 1:3656885/71‑1 (MQ=255) cacGCCGTCATCGTACCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGggtgg < 1:560868/71‑1 (MQ=255) acGCCGTCATCGTCCCATCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGaa < 1:1673405/63‑1 (MQ=255) acGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGaa < 1:1024009/63‑1 (MQ=255) acGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGaa < 1:2335303/63‑1 (MQ=255) acGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGaa < 1:3338224/63‑1 (MQ=255) ccGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGtttt < 1:1395218/71‑1 (MQ=255) cGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGt > 1:3481566/1‑44 (MQ=255) cGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGt > 1:398567/1‑44 (MQ=255) cGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTa > 1:649091/1‑71 (MQ=255) tCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTAc < 1:1303366/70‑1 (MQ=255) cATCGTCCCAGCTGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCg > 1:531925/1‑71 (MQ=255) cGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCg > 1:1920615/1‑68 (MQ=255) gTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGtttt < 1:343695/63‑1 (MQ=255) gTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTg < 1:2104026/70‑1 (MQ=255) tCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGcc > 1:903739/1‑71 (MQ=255) cccAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTg < 1:3589536/68‑1 (MQ=255) cccAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCg > 1:1522498/1‑71 (MQ=255) cccAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCg > 1:3462469/1‑71 (MQ=255) cccAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCg > 1:3138598/1‑71 (MQ=255) cAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCGGa < 1:2886926/71‑1 (MQ=255) cAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCGGa < 1:1128329/71‑1 (MQ=255) aGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCGGaa < 1:1604196/71‑1 (MQ=255) gCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGttt < 1:492865/56‑1 (MQ=255) gCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGttt < 1:100536/56‑1 (MQ=255) gCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTAc > 1:392555/1‑59 (MQ=255) gCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTAc > 1:1519290/1‑59 (MQ=255) | CTTTCGACAGCTTGATAGTTTTTGCGTAGCAGCCGCCTTCGAAGTTAAACACGCCGTCATCGTCCCAGCCGTGTTCGTCATCGCCAATCAGGCGACGTTTCGGGTCGGTGGAAAGGGTGGTTTTACCGGTGCCGGAA > minE/2687396‑2687532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |