| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,708,236 | A→G | 8.1% | E260G (GAA→GGA) | trpS → | tryptophanyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,708,236 | 0 | A | G | 8.1% | 210.4 / 8.6 | 74 | E260G (GAA→GGA) | trpS | tryptophanyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (34/34); new base G (4/2); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TGCAGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTCCGG > minE/2708167‑2708305 | tgCAGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGaa < 1:1213054/71‑1 (MQ=255)tgCAGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGa < 1:130615/70‑1 (MQ=255) cAGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAAc < 1:1034607/70‑1 (MQ=255) aGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTg > 1:983569/1‑71 (MQ=255) aGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTg > 1:3145158/1‑71 (MQ=255) aGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTg < 1:1901613/71‑1 (MQ=255) aaaGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGaaa > 1:3290736/1‑70 (MQ=255) aaaGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGaaa > 1:2149361/1‑70 (MQ=255) gggCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGt > 1:1665954/1‑71 (MQ=255) gcttCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:2795609/69‑1 (MQ=255) gTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:2764709/71‑1 (MQ=255) gTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:1494866/71‑1 (MQ=255) tttCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg > 1:479958/1‑69 (MQ=255) tttCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:509267/70‑1 (MQ=255) tCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:3459032/1‑71 (MQ=255) tCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:1357172/1‑71 (MQ=255) ccAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:2518154/1‑70 (MQ=255) ccAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:693640/1‑70 (MQ=255) ccAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:1825316/1‑70 (MQ=255) ccAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAgg > 1:1708233/1‑70 (MQ=255) cAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg < 1:2044377/65‑1 (MQ=255) cAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg < 1:584052/65‑1 (MQ=255) cAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:1709842/66‑1 (MQ=255) cAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAAAAGTTCg < 1:475840/65‑1 (MQ=255) aaCCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGc < 1:2953155/69‑1 (MQ=255) ccTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg > 1:1788080/1‑62 (MQ=255) ccTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg > 1:838232/1‑62 (MQ=255) ccTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCg > 1:56788/1‑62 (MQ=255) cTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGAt < 1:1823834/71‑1 (MQ=255) ttGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTa < 1:495728/71‑1 (MQ=255) ggATATCCTTTCAGCGGTAACTGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATg < 1:942717/67‑1 (MQ=255) ggATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATg > 1:500508/1‑71 (MQ=255) atatCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGt > 1:283013/1‑71 (MQ=255) tatCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:2290305/54‑1 (MQ=255) atCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGaa < 1:1996312/36‑1 (MQ=255) atCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGaa < 1:1252592/36‑1 (MQ=255) atCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGaa < 1:719729/36‑1 (MQ=255) atCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGaa < 1:500451/36‑1 (MQ=255) atCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGt < 1:1339735/49‑1 (MQ=255) ccTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACt > 1:3436270/1‑36 (MQ=255) ccTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACt > 1:3152181/1‑36 (MQ=255) ccTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACt > 1:3032863/1‑36 (MQ=255) ccTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTa < 1:2518012/63‑1 (MQ=255) tttCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGaaa < 1:3232510/39‑1 (MQ=255) tttCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGaaa < 1:3460926/39‑1 (MQ=255) tCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGa > 1:3434862/1‑55 (MQ=255) tCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGa > 1:1904373/1‑55 (MQ=255) tCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGa > 1:185323/1‑70 (MQ=255) cAGCGGTAACGGGCCAGAGCATCCCAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTc < 1:91488/63‑1 (MQ=255) gCGGTAACGGGCCAGAGCATCCCAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCAt > 1:3251881/1‑63 (MQ=255) ggTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTg < 1:1994403/71‑1 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGa < 1:1617215/40‑1 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGa < 1:3594495/63‑1 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGa < 1:2446589/63‑1 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTg < 1:3617810/69‑1 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGa > 1:3017153/1‑70 (MQ=255) tAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGa > 1:133534/1‑70 (MQ=255) aaCGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGa < 1:2161763/62‑1 (MQ=255) aCGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGt > 1:753183/1‑71 (MQ=255) gggCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTgg > 1:1230172/1‑71 (MQ=255) ggCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATc > 1:832847/1‑55 (MQ=255) ggCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGc < 1:2947250/71‑1 (MQ=255) gCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGc > 1:1264733/1‑70 (MQ=255) gCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGc > 1:593155/1‑70 (MQ=255) ccAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTa > 1:1893730/1‑45 (MQ=255) cAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCt < 1:377297/53‑1 (MQ=255) cAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCt < 1:2353415/53‑1 (MQ=255) cAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGa > 1:3276232/1‑71 (MQ=255) cAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGAGGCTGa < 1:481372/71‑1 (MQ=255) agagCATCCCAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGAt > 1:1920253/1‑71 (MQ=255) gagCATCCCAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATg > 1:292972/1‑71 (MQ=255) gagCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATg > 1:1184822/1‑71 (MQ=255) agCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGc > 1:1100736/1‑71 (MQ=255) cccAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTg < 1:1116429/53‑1 (MQ=255) ccAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc < 1:1907137/71‑1 (MQ=255) ccAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc < 1:1191610/71‑1 (MQ=255) ccAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc < 1:84073/71‑1 (MQ=255) ccAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc < 1:1496687/71‑1 (MQ=255) cAGGACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGttt < 1:3435081/69‑1 (MQ=255) cAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGc > 1:3055167/1‑64 (MQ=255) aaCTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc > 1:2112057/1‑67 (MQ=255) aaCTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTc > 1:3737484/1‑67 (MQ=255) aaCTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTCCgg > 1:142688/1‑70 (MQ=255) | TGCAGAACAAAGCGGGCGTTTCCAACCTGTTGGATATCCTTTCAGCGGTAACGGGCCAGAGCATCCCAGAACTGGAAAAACAGTTCGAAGGCAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTCCGG > minE/2708167‑2708305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |