| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,727,940 | T→C | 60.5% | F59F (TTT→TTC) | kefB → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,727,940 | 0 | T | C | 60.5% | 29.2 / 45.8 | 43 | F59F (TTT→TTC) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (9/8); new base C (10/16); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTG > minE/2727872‑2728005 | cTGTCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACtttt < 1:389767/70‑1 (MQ=255)cTGGCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTc < 1:748244/71‑1 (MQ=255) gCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCgg < 1:497290/70‑1 (MQ=255) aGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:202506/71‑1 (MQ=255) aGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAAc < 1:3426712/71‑1 (MQ=255) aGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAAc > 1:2151201/1‑71 (MQ=255) gggATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACt < 1:2973771/71‑1 (MQ=255) ggATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTc > 1:1236214/1‑71 (MQ=255) tGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCg > 1:645429/1‑71 (MQ=255) gCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:3365483/70‑1 (MQ=255) gCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCg < 1:1364845/70‑1 (MQ=255) tGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTAt > 1:135535/1‑71 (MQ=255) gCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTc > 1:3681987/1‑71 (MQ=255) gggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg > 1:925179/1‑71 (MQ=255) gggggCTAGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg > 1:349930/1‑71 (MQ=255) ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGt < 1:102285/71‑1 (MQ=255) ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCgg < 1:836928/45‑1 (MQ=255) tGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCgg < 1:3264345/49‑1 (MQ=255) tGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCgg < 1:2705634/49‑1 (MQ=255) tGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCgg < 1:1216483/49‑1 (MQ=255) ggTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTatcatc < 1:2586353/71‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCgg < 1:1052473/44‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCgg < 1:3225462/44‑1 (MQ=255) tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAAc > 1:2668325/1‑38 (MQ=255) tattaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGcc > 1:904082/1‑71 (MQ=255) ttaGCGACGTCGATGAGATCCTCCACTTCTAGCAACTCGGCGTGGTATTCCTg > 1:2341479/1‑53 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:2331147/1‑70 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:3015455/1‑70 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCg < 1:2535145/41‑1 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTgg > 1:2371195/1‑44 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTgg > 1:1837547/1‑44 (MQ=255) aCGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTg < 1:1786630/71‑1 (MQ=255) gTCGATGAGATCCTGCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa < 1:2905342/71‑1 (MQ=255) gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGa > 1:3593491/1‑70 (MQ=255) tCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:3212610/1‑62 (MQ=255) gATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATc < 1:1910112/70‑1 (MQ=255) agaTCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTc > 1:2972636/1‑70 (MQ=255) gaTCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCa < 1:2927323/71‑1 (MQ=255) cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAAc > 1:1772147/1‑71 (MQ=255) cACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACtt < 1:986356/69‑1 (MQ=255) cACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACtt < 1:2306706/69‑1 (MQ=255) cACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACtt < 1:1180362/69‑1 (MQ=255) cACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCATCCAAACttt < 1:2271121/70‑1 (MQ=255) cTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTg > 1:2679769/1‑69 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCaaa > 1:2216570/1‑62 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCaaa > 1:2199057/1‑62 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCaaa > 1:944740/1‑62 (MQ=255) | CTGGCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTG > minE/2727872‑2728005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |