| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 288,729 | A→T | 5.6% | E458V (GAA→GTA) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 288,729 | 0 | A | T | 5.6% | 362.5 / 8.6 | 122 | E458V (GAA→GTA) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (59/56); new base T (3/4); total (62/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTG > minE/288662‑288799 | ccGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGt > 1:1809984/1‑71 (MQ=255) cGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGAAAGt < 1:1799857/70‑1 (MQ=255) ctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggt < 1:263352/69‑2 (MQ=255) ctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggt < 1:1656018/69‑2 (MQ=255) ctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggtcg > 1:715264/1‑66 (MQ=38) ctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggtcg > 1:2268091/1‑66 (MQ=38) ctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggtcg > 1:270446/1‑66 (MQ=38) cACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACg < 1:435618/69‑1 (MQ=255) cTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGAtct < 1:248960/71‑1 (MQ=255) cTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGAtct < 1:1980611/71‑1 (MQ=255) tGCTTGAAGTGCTGGATCCAGAGCAGAATGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGAtct > 1:162841/1‑70 (MQ=255) aGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACg < 1:957546/71‑1 (MQ=255) aGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACg < 1:1011376/71‑1 (MQ=255) aGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACg < 1:1489812/71‑1 (MQ=255) gTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGt < 1:309700/71‑1 (MQ=255) gTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGt < 1:2090850/71‑1 (MQ=255) tGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGt > 1:1570036/1‑70 (MQ=255) cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGAt > 1:3728884/1‑71 (MQ=255) cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGAt > 1:3605419/1‑71 (MQ=255) cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGAt > 1:1897685/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt > 1:3097959/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt > 1:3434322/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt > 1:3148970/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt > 1:3496089/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt > 1:1714986/1‑71 (MQ=255) gATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGt < 1:2970246/70‑1 (MQ=255) aTCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGt < 1:236178/69‑1 (MQ=255) tCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg < 1:2600358/71‑1 (MQ=255) cAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGt < 1:2559638/70‑1 (MQ=255) cAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTc > 1:2131936/1‑71 (MQ=255) agagCAGAACGTAGCGTTCAGCGACCACTTCCAGGTAGTggt < 1:1331399/42‑2 (MQ=25) gagCAGAACGTAGCGTTCAGCGACCACTACCTGGTAGTGGATTACGATCTCAGCGACGTGATGt < 1:2941984/64‑1 (MQ=255) gagCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGt < 1:3530521/64‑1 (MQ=255) gagCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGt < 1:314766/64‑1 (MQ=255) gagCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGt > 1:2389418/1‑68 (MQ=255) gagCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGt > 1:1470581/1‑68 (MQ=255) gCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt > 1:3307799/1‑64 (MQ=255) gCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa > 1:3489867/1‑71 (MQ=255) cAGAACTTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTg > 1:7383/1‑57 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTg > 1:2533156/1‑57 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTg > 1:2529059/1‑57 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTg > 1:3339815/1‑57 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTg > 1:225053/1‑57 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc < 1:837256/71‑1 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc > 1:3687133/1‑71 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc < 1:1684620/71‑1 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc < 1:198546/71‑1 (MQ=255) cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc < 1:1985673/71‑1 (MQ=255) aGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACg < 1:2455930/71‑1 (MQ=255) gAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt > 1:2481253/1‑71 (MQ=255) gAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt < 1:3639557/71‑1 (MQ=255) gAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt > 1:3573963/1‑71 (MQ=255) gAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt > 1:1689513/1‑71 (MQ=255) gAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt < 1:3255065/71‑1 (MQ=255) aCGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCg < 1:3568150/71‑1 (MQ=255) gCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTc < 1:488267/71‑1 (MQ=255) cGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTc > 1:1087323/1‑70 (MQ=255) cGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTc > 1:2371012/1‑70 (MQ=255) gTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTcc < 1:2157796/70‑1 (MQ=255) ttCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACg < 1:2739716/60‑1 (MQ=255) ttCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACg < 1:462747/60‑1 (MQ=255) ttCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCAt < 1:2308193/71‑1 (MQ=255) tCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTcc > 1:865482/1‑68 (MQ=255) tCAGCGACCACTACCTGGAAGTGGATTACGATCTAAGCGACGTGATGTTTGTCGCGACGTCGAACTcc > 1:633998/1‑68 (MQ=255) cAGCTACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATg < 1:2161602/46‑1 (MQ=255) cAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATg < 1:2703085/46‑1 (MQ=255) cAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATg < 1:1529337/46‑1 (MQ=255) cAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATg > 1:2321913/1‑70 (MQ=255) cAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATg < 1:3428254/70‑1 (MQ=255) aGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa > 1:234160/1‑55 (MQ=255) aGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa > 1:1383863/1‑55 (MQ=255) gCGACCACTACCTGTAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAAc < 1:145716/62‑1 (MQ=255) gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg < 1:2388214/48‑1 (MQ=255) gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg < 1:2914004/48‑1 (MQ=255) gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAAc < 1:2783438/62‑1 (MQ=255) gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAAc < 1:754069/62‑1 (MQ=255) gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGaa > 1:2264817/1‑70 (MQ=255) cGACCACTACCTGGAAGTGGATTACGATCTCAGCGa > 1:517040/1‑36 (MQ=255) gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAt > 1:754249/1‑71 (MQ=255) gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAt > 1:3648463/1‑71 (MQ=255) gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAt < 1:2490446/71‑1 (MQ=255) gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAt > 1:1285453/1‑71 (MQ=255) aCCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt < 1:1970709/43‑1 (MQ=255) aCCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAtt > 1:862534/1‑71 (MQ=255) ccACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTc < 1:125309/71‑1 (MQ=255) ccACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTc > 1:225168/1‑71 (MQ=255) cACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTg > 1:2873051/1‑43 (MQ=255) cACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCg < 1:2503184/54‑1 (MQ=255) cACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCg < 1:3254447/54‑1 (MQ=255) cACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTcc < 1:2726428/71‑1 (MQ=255) aCTACCTGGAAGTGGATTACGATCTCAGCGACGTGATg < 1:1563380/38‑1 (MQ=255) tACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCgg > 1:116113/1‑70 (MQ=255) tACCTGGAAGTGGATTACGATCTCAGCGAAGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCgg > 1:421123/1‑70 (MQ=255) ccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcg > 1:526434/1‑41 (MQ=255) ccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcg > 1:1374110/1‑41 (MQ=255) ccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcg > 1:2303938/1‑41 (MQ=255) ccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCa < 1:2407534/70‑1 (MQ=255) ccTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCa < 1:2510541/70‑1 (MQ=255) ggAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc > 1:427033/1‑71 (MQ=255) gAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgct < 1:1312125/71‑1 (MQ=255) | CCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTG > minE/288662‑288799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |