| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,852,263 | T→A | 9.4% | K303N (AAA→AAT) | rsgA ← | ribosome small subunit‑dependent GTPase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,852,263 | 0 | T | A | 9.4% | 180.9 / 9.6 | 64 | K303N (AAA→AAT) | rsgA | ribosome small subunit‑dependent GTPase A |
| Reads supporting (aligned to +/- strand): ref base T (36/22); new base A (2/4); total (38/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGTTCC > minE/2852193‑2852332 | cGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCgtgt > 1:540971/1‑71 (MQ=255) aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTg > 1:1520900/1‑70 (MQ=255) aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGc < 1:3528921/71‑1 (MQ=255) aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGc < 1:3533269/71‑1 (MQ=255) gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa < 1:536184/71‑1 (MQ=255) gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa < 1:404548/71‑1 (MQ=255) gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa < 1:394875/71‑1 (MQ=255) tttCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAAt < 1:1860923/69‑1 (MQ=255) tttCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAAt < 1:470501/69‑1 (MQ=255) cgcgATTTTCCCTTACTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGat < 1:509343/71‑1 (MQ=255) gATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt > 1:669150/1‑71 (MQ=255) ttttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAttt > 1:1765263/1‑70 (MQ=255) ttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTg > 1:280034/1‑54 (MQ=255) ttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATc > 1:1534235/1‑59 (MQ=255) tCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTaca < 1:953887/70‑1 (MQ=255) ccTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTAcaca < 1:1918157/70‑1 (MQ=255) cTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt < 1:3677075/63‑1 (MQ=255) cTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt < 1:2885649/63‑1 (MQ=255) ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa > 1:3486802/1‑52 (MQ=255) ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa > 1:3193652/1‑52 (MQ=255) ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa > 1:218970/1‑52 (MQ=255) tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATGGTGTTTGCAATCGCGATATTTACACAGAcc > 1:62465/1‑71 (MQ=255) tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGatat > 1:1255918/1‑60 (MQ=255) tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTa > 1:2514641/1‑63 (MQ=255) ccTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGAcc > 1:1516278/1‑70 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCt < 1:2318806/70‑1 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa < 1:1726579/71‑1 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa < 1:816498/71‑1 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa < 1:2742110/71‑1 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa < 1:2686342/71‑1 (MQ=255) cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa < 1:886755/71‑1 (MQ=255) aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:3438348/1‑70 (MQ=255) aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:3057364/1‑70 (MQ=255) aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:3142763/1‑70 (MQ=255) aCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:3222504/1‑69 (MQ=255) aCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:309502/1‑69 (MQ=255) cTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCa > 1:3059887/1‑71 (MQ=255) cccGGATAGCGCAGCCCGGATCGGTATCGTCTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATg > 1:2482006/1‑70 (MQ=255) cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat < 1:520680/45‑1 (MQ=17) cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat < 1:581387/45‑1 (MQ=17) cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat < 1:1957072/45‑1 (MQ=17) cccGGATAGCGCAGCCCGGATCGATATCGCGATAGCAAACACGat < 1:64711/45‑1 (MQ=12) ccGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATgg < 1:370204/70‑1 (MQ=255) cGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGatac > 1:3719780/1‑44 (MQ=9) cGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGatac > 1:2481723/1‑44 (MQ=9) aTAGCGGAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:478954/1‑57 (MQ=255) aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa > 1:1517284/1‑57 (MQ=255) aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTc < 1:2735768/62‑1 (MQ=255) aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTc < 1:1821846/62‑1 (MQ=255) aGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa > 1:3160072/1‑71 (MQ=255) cGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt > 1:2026721/1‑71 (MQ=255) cGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt > 1:1819061/1‑71 (MQ=255) gATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt > 1:2752428/1‑69 (MQ=255) gATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt > 1:3293946/1‑69 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACATACCTAAATAGTCATGGAATTCGACAAAGCCCTGAg < 1:390681/71‑1 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACaaa > 1:2872075/1‑63 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc > 1:2059711/1‑67 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc > 1:287005/1‑67 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc > 1:1081180/1‑67 (MQ=255) aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc > 1:1657178/1‑67 (MQ=255) ggTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGa > 1:251895/1‑71 (MQ=255) aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt > 1:894164/1‑71 (MQ=255) aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt > 1:1947891/1‑71 (MQ=255) aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt > 1:206698/1‑71 (MQ=255) aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt > 1:232565/1‑71 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAAt > 1:2329464/1‑46 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTc > 1:1779667/1‑48 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTc > 1:230874/1‑48 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa > 1:572891/1‑50 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa > 1:794823/1‑50 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa > 1:2852664/1‑50 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGtt < 1:1758270/71‑1 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGt > 1:409249/1‑70 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTAGa > 1:1485525/1‑50 (MQ=255) gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATAGAATTCGACAAAGCCCTGAGTGATTTGt < 1:3554812/70‑1 (MQ=255) gtgtTTGCAATCGAGATATTTACACAGACCTAAATAGTCATGGAATTCGa > 1:1488903/1‑50 (MQ=255) tttGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGTTcc < 1:1408466/70‑1 (MQ=255) | CGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGTTCC > minE/2852193‑2852332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |