| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,892,177 | G→A | 47.6% | D278N (GAC→AAC) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,892,177 | 0 | G | A | 47.6% | 8.4 / 87.3 | 63 | D278N (GAC→AAC) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/16); new base A (21/9); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC > minE/2892111‑2892242 | gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGACGAATATGGCAGGTCCCGCCTTCAAcgc > 1:253698/1‑71 (MQ=255) gatgatGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCAAATATGGCAGGTCCCGCCTTCGACgcgc < 1:1458203/71‑1 (MQ=255) gatggatgGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTg > 1:2263692/1‑70 (MQ=255) tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:3170661/71‑1 (MQ=255) tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2950030/71‑1 (MQ=255) tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2722233/71‑1 (MQ=255) tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2312324/71‑1 (MQ=255) gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga > 1:3109195/1‑70 (MQ=255) gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga > 1:1016659/1‑70 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGTTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1062231/70‑1 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:3466392/70‑1 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1036881/70‑1 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1723644/70‑1 (MQ=255) gaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACa > 1:383313/1‑70 (MQ=255) agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaa > 1:3735498/1‑70 (MQ=255) agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa > 1:3716202/1‑70 (MQ=255) gcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaa < 1:3578902/71‑1 (MQ=255) gaaCCCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa > 1:3252216/1‑68 (MQ=255) aCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGAc > 1:1028507/1‑71 (MQ=255) cGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACg > 1:900112/1‑71 (MQ=255) cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:487186/1‑71 (MQ=255) cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:371540/1‑71 (MQ=255) aaGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:2622618/2‑71 (MQ=255) aGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc < 1:1465471/71‑1 (MQ=255) gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:1022778/1‑70 (MQ=255) gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2944146/1‑70 (MQ=255) gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2757459/1‑70 (MQ=255) gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2419946/1‑70 (MQ=255) tgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATAc < 1:1588034/55‑1 (MQ=255) ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGATAAATACAAAAAAGACGGCACCAt < 1:1927494/70‑1 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGc > 1:2199687/1‑70 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAAt > 1:3733289/1‑49 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:114306/1‑71 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:2829525/1‑71 (MQ=255) aCTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt > 1:2616260/1‑70 (MQ=255) cTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:155899/1‑68 (MQ=255) aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa > 1:1359732/1‑51 (MQ=255) aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa > 1:143461/1‑51 (MQ=255) aCGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa < 1:602871/69‑1 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaaaaa > 1:1591633/1‑49 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:2686568/1‑71 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:457196/1‑71 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:2507619/1‑71 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:3516704/1‑71 (MQ=255) ccGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCt < 1:2175328/71‑1 (MQ=255) gAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:16642/1‑71 (MQ=255) tatGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt < 1:169628/71‑1 (MQ=255) tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt < 1:962460/70‑1 (MQ=255) tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa < 1:2248280/71‑1 (MQ=255) gCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTaa < 1:3676005/70‑1 (MQ=255) ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc > 1:1770742/1‑69 (MQ=255) ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa > 1:2413012/1‑70 (MQ=255) gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa < 1:2890479/71‑1 (MQ=255) gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa < 1:2326991/71‑1 (MQ=255) cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGAc > 1:1794505/1‑59 (MQ=255) cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg > 1:2261450/1‑60 (MQ=255) cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg > 1:1722575/1‑60 (MQ=255) ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa > 1:3190382/1‑52 (MQ=255) ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa > 1:435538/1‑52 (MQ=255) ccGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc < 1:2360818/70‑1 (MQ=255) gCCTTCGACGCGCTGGAGAAAAACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATacac < 1:1675265/71‑1 (MQ=255) ccTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAcc < 1:2498058/71‑1 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCAc < 1:558214/35‑1 (MQ=255) | GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC > minE/2892111‑2892242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |