| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 318,760 | G→C | 7.1% | T174T (ACC→ACG) | priC ← | primosomal replication protein N'' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 318,760 | 0 | G | C | 7.1% | 215.2 / 5.7 | 70 | T174T (ACC→ACG) | priC | primosomal replication protein N'' |
| Reads supporting (aligned to +/- strand): ref base G (31/34); new base C (2/3); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGG > minE/318690‑318830 | aaTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCgg > 1:2326364/1‑71 (MQ=255) tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCggg < 1:2564352/70‑1 (MQ=255) tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCggg < 1:2463052/70‑1 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt < 1:352840/70‑1 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt < 1:3182961/70‑1 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt > 1:1846686/1‑70 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt > 1:55408/1‑70 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt > 1:741730/1‑70 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGtt > 1:900728/1‑70 (MQ=255) aaaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGt > 1:49311/1‑69 (MQ=255) tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAc > 1:2207046/1‑71 (MQ=255) gACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcgc > 1:712357/1‑71 (MQ=255) gACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcgc > 1:2247664/1‑71 (MQ=255) ccAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTaa > 1:161489/1‑71 (MQ=255) cAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGCGTTTAACCCGCTag < 1:2131568/70‑2 (MQ=38) aGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAcc > 1:3291133/1‑71 (MQ=255) aGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAcc > 1:3314044/1‑71 (MQ=255) tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTg < 1:3119934/71‑1 (MQ=255) tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTg < 1:1116303/71‑1 (MQ=255) tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTg < 1:2024418/71‑1 (MQ=255) tGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGtt > 1:1967934/1‑71 (MQ=255) gACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTa < 1:555045/63‑1 (MQ=255) gACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTa < 1:2225937/63‑1 (MQ=255) aCATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcg < 1:1544715/57‑1 (MQ=255) cATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcg < 1:2377913/56‑1 (MQ=255) cATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTc < 1:1379801/71‑1 (MQ=255) cATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTc > 1:1722771/1‑71 (MQ=255) aTCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCg < 1:3530937/71‑1 (MQ=255) aTCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCg < 1:1079709/71‑1 (MQ=255) tCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGCGttta < 1:3405436/51‑3 (MQ=255) cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGCGttta < 1:491615/50‑3 (MQ=255) gTCAGGGGGATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTaaa > 1:1040370/1‑50 (MQ=255) gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGCGTTTAAc > 1:925117/1‑51 (MQ=255) gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGCGTTTAAc > 1:3280941/1‑51 (MQ=255) gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTaaa > 1:2484134/1‑50 (MQ=255) gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTaa > 1:1567417/1‑49 (MQ=255) tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAttt > 1:591725/1‑71 (MQ=255) gggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc < 1:3264525/71‑1 (MQ=255) ggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTa < 1:2523465/42‑1 (MQ=255) ggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTa < 1:62452/42‑1 (MQ=255) ggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTc < 1:1683747/61‑1 (MQ=255) ggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTc < 1:2771113/61‑1 (MQ=255) gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc > 1:3424086/1‑69 (MQ=255) gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc > 1:2181712/1‑69 (MQ=255) gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc > 1:1551120/1‑69 (MQ=255) cATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGa < 1:1931461/61‑1 (MQ=255) aTTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgc > 1:2765640/1‑71 (MQ=255) ttttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc < 1:842226/66‑1 (MQ=255) ttttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgcg < 1:1422568/71‑1 (MQ=255) cAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTTTTTTCg < 1:480709/53‑1 (MQ=255) cAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCg < 1:1457404/53‑1 (MQ=255) cAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAAGCGCTAACCTGTTTTCg < 1:3487688/53‑1 (MQ=255) aGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggc < 1:3516549/71‑1 (MQ=255) gTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcg > 1:2890849/1‑71 (MQ=255) gTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcg < 1:312023/71‑1 (MQ=255) tGATATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg > 1:2489485/1‑71 (MQ=255) tGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg > 1:2456053/1‑71 (MQ=255) tGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcg > 1:1421924/1‑70 (MQ=255) atatTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGc < 1:3174203/71‑1 (MQ=255) ttCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGt < 1:3376003/70‑1 (MQ=255) ttCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGt < 1:2952291/70‑1 (MQ=255) ttCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTg < 1:2285052/71‑1 (MQ=255) tctctcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTcc > 1:3551980/1‑51 (MQ=255) ctcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg > 1:517386/1‑71 (MQ=255) tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCa < 1:2338513/69‑1 (MQ=255) ccATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgc > 1:1563195/1‑71 (MQ=255) cATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg > 1:3249197/1‑47 (MQ=255) cATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg > 1:612055/1‑47 (MQ=255) ttGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc < 1:1204668/42‑1 (MQ=255) ttGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc < 1:3642031/42‑1 (MQ=255) tGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcgc > 1:2667413/1‑71 (MQ=255) gCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcgc < 1:1225058/70‑1 (MQ=255) gCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTc < 1:2950062/69‑1 (MQ=255) gggTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAg > 1:3041466/1‑71 (MQ=255) ggTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAgg > 1:450522/1‑71 (MQ=255) | AATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGG > minE/318690‑318830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |