| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 30,585 | T→C | 5.3% | A697A (GCT→GCC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 30,585 | 0 | T | C | 5.3% | 224.6 / 2.4 | 75 | A697A (GCT→GCC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (42/29); new base C (2/2); total (44/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGTT > minE/30520‑30651 | gAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTg > 1:2824719/1‑70 (MQ=255) aaCGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGa > 1:2666215/1‑70 (MQ=255) cGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt < 1:319541/71‑1 (MQ=255) cGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt < 1:2097868/71‑1 (MQ=255) tCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg > 1:1367575/1‑71 (MQ=255) ccAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGc < 1:1780840/69‑1 (MQ=255) aGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCgg > 1:1759100/1‑69 (MQ=255) aGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCgg > 1:2983234/1‑69 (MQ=255) aGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCgg > 1:2007545/1‑69 (MQ=255) aGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCCATTTCAATAGCGGTa < 1:1102557/71‑1 (MQ=38) cATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt < 1:2485369/68‑1 (MQ=255) tGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa > 1:3490802/1‑59 (MQ=255) tGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCCAtt < 1:1192170/55‑1 (MQ=255) cGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa < 1:1901914/70‑1 (MQ=255) cGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa < 1:430150/70‑1 (MQ=255) cGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa < 1:3527306/70‑1 (MQ=255) cGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg > 1:3121813/1‑71 (MQ=255) gTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGa < 1:3074387/53‑1 (MQ=255) gTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGa < 1:1492006/53‑1 (MQ=255) gTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGc > 1:1893304/1‑71 (MQ=255) tGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCTCTATTGAAATGGCGGTAGAGAAGGCg > 1:1436864/1‑70 (MQ=255) tGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa > 1:311488/1‑53 (MQ=255) tGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa > 1:3391404/1‑53 (MQ=255) tGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCg > 1:1930440/1‑70 (MQ=255) gAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa > 1:1703484/1‑70 (MQ=255) gAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa < 1:362450/70‑1 (MQ=255) aGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTCAAATGGCGGTAGAGAAGGCGaaa < 1:3419616/71‑1 (MQ=255) cGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAaga < 1:2871990/71‑1 (MQ=255) aaactgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt < 1:1635377/69‑1 (MQ=255) aactgaaacAACCGGCGAAGGCCACCGTTACCGCTAtt < 1:1178863/38‑1 (MQ=255) aactgaaacAACCGGCGAACGCCACCGTTACCGCTAtt < 1:1566597/38‑1 (MQ=255) aactgaaacAACCGGCGAACGCCACCGTTACCGCTAtt < 1:3336301/38‑1 (MQ=255) aactgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg > 1:2566418/1‑47 (MQ=255) actgaaacAATCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga > 1:2825473/1‑53 (MQ=255) actgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTagag > 1:1538067/1‑52 (MQ=255) actgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga > 1:1586421/1‑53 (MQ=255) actgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga > 1:1991013/1‑53 (MQ=255) actgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga > 1:3274857/1‑53 (MQ=255) actgaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga > 1:969532/1‑53 (MQ=255) gaaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt < 1:1607023/63‑1 (MQ=255) aaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg < 1:1985152/51‑1 (MQ=255) aaacAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg < 1:760884/51‑1 (MQ=255) aacaacCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAgg > 1:1836599/1‑51 (MQ=255) aacaacCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAgg > 1:1832257/1‑51 (MQ=255) acaacCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGc > 1:1149932/1‑71 (MQ=255) aacCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCtg > 1:978328/1‑71 (MQ=255) aacCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCtg > 1:1022847/1‑71 (MQ=255) ccGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCtggt > 1:3178333/1‑71 (MQ=255) ccGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCtggt > 1:2345921/1‑71 (MQ=255) ccGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCtggt < 1:401066/71‑1 (MQ=255) cGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTggtggt < 1:1555689/70‑1 (MQ=255) cGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTa > 1:1229980/1‑71 (MQ=255) cGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTa > 1:2030174/1‑71 (MQ=255) gAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTAc < 1:2808853/71‑1 (MQ=255) cGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTAcgtc > 1:3177858/1‑71 (MQ=255) ccACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTAcgtccg > 1:2636021/1‑71 (MQ=255) aCCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTAcgtccgt > 1:2672637/1‑70 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGc > 1:282335/1‑47 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGc > 1:3701454/1‑47 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGc > 1:497190/1‑47 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCt > 1:1861610/1‑71 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCt > 1:3304867/1‑71 (MQ=255) ccGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCt > 1:3300479/1‑71 (MQ=255) cGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCtt < 1:1875636/71‑1 (MQ=255) gTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTa < 1:787510/71‑1 (MQ=255) ctACCGCCATTTCAATAGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTAc > 1:307191/2‑71 (MQ=37) ctACCGCCATTTCAATAGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTAc > 1:1550336/2‑71 (MQ=37) aCCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGt > 1:1509957/1‑71 (MQ=255) aCCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGt > 1:137588/1‑71 (MQ=255) ccGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGtt < 1:194588/71‑1 (MQ=255) ccGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGtt < 1:3384121/71‑1 (MQ=255) ccGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGtt < 1:796487/71‑1 (MQ=255) ccGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGtt < 1:2169646/71‑1 (MQ=255) cGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCt < 1:598681/48‑1 (MQ=255) cTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCt > 1:104830/1‑39 (MQ=255) | GAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACCCGCTGGTGGTACGTCCGTCTTACGTT > minE/30520‑30651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |