| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 359,417 | G→A | 13.5% | G216S (GGT→AGT) | sfmD → | predicted outer membrane export usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 359,417 | 0 | G | A | 13.5% | 161.5 / 14.9 | 59 | G216S (GGT→AGT) | sfmD | predicted outer membrane export usher protein |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base A (6/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGG > minE/359349‑359482 | gTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATgg < 1:65021/70‑1 (MQ=255)gTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGt < 1:3421123/71‑1 (MQ=255) aCGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTc < 1:321655/70‑1 (MQ=255) cGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCt > 1:939703/1‑71 (MQ=255) cGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCt < 1:47867/71‑1 (MQ=255) gATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTg > 1:3454605/1‑71 (MQ=255) aTAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGATCCTg > 1:79117/1‑70 (MQ=255) tttttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTAtt > 1:3290794/1‑71 (MQ=255) tttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATAGTTCCAGGAACCAttg < 1:2501335/70‑2 (MQ=38) tttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATAGTTCCAGGAACCAttg < 1:1843514/70‑2 (MQ=38) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTgg > 1:2324704/1‑60 (MQ=255) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc > 1:1482658/1‑70 (MQ=255) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATAGTTCCAgg > 1:282727/1‑60 (MQ=255) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATAGTTCCAGGAACCAttg > 1:2527780/1‑67 (MQ=38) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATAGTTCCAGGAACCAttg > 1:265835/1‑67 (MQ=38) ttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcg < 1:1751062/70‑1 (MQ=255) ttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcg < 1:3017912/70‑1 (MQ=255) aGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGaaa < 1:3528974/70‑1 (MQ=255) aGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAAt < 1:1328461/71‑1 (MQ=255) gCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATg < 1:1385374/71‑1 (MQ=255) gCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATg > 1:2060088/1‑71 (MQ=255) gCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATg < 1:3262091/66‑1 (MQ=255) cGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGatat > 1:3182397/1‑70 (MQ=255) cGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGatat > 1:2430152/1‑70 (MQ=255) gggATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCa < 1:939532/71‑1 (MQ=255) gggATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCa > 1:690946/1‑71 (MQ=255) gggATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCa > 1:1416540/1‑71 (MQ=255) gggATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCa < 1:1835197/71‑1 (MQ=255) aTTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTc < 1:2561643/71‑1 (MQ=255) aaTATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAg < 1:1291712/70‑1 (MQ=255) aaTATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAg > 1:1800028/1‑70 (MQ=255) atTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGaa > 1:3283148/1‑69 (MQ=255) ttGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc > 1:3544505/1‑45 (MQ=255) ttGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAAc < 1:1600020/69‑1 (MQ=255) ttGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAg > 1:3273700/1‑71 (MQ=255) cccGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGa < 1:3145295/71‑1 (MQ=255) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaa > 1:1326734/1‑71 (MQ=255) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaa < 1:1708034/71‑1 (MQ=255) cGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat < 1:784800/71‑1 (MQ=255) cGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat < 1:772477/71‑1 (MQ=255) gTGGCGTTTACGCAACAATGGTTCTTGGAACTAtttt > 1:1514068/1‑37 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat > 1:1404443/1‑70 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat > 1:829994/1‑70 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat > 1:1260541/1‑70 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat > 1:2534419/1‑70 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaa > 1:287779/1‑69 (MQ=255) tGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcgcg < 1:415405/40‑1 (MQ=255) tGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataa < 1:3075361/71‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATTGATATCATTCAGAACAGTTGAataata < 1:3328808/71‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataat < 1:349478/70‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataat < 1:729915/70‑1 (MQ=255) catttCCGCGAAAATAGTTCCAGGAACCATTGTTGCGGAAATGGATATCATTCAGAACAGTGGAATAatat > 1:2465091/3‑71 (MQ=9) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATAtt > 1:1559657/1‑68 (MQ=255) aCGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCa > 1:1140651/1‑71 (MQ=255) aacaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTg > 1:2494508/1‑71 (MQ=255) acaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTgg < 1:605177/71‑1 (MQ=255) acaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTgg < 1:2754335/71‑1 (MQ=255) aaaTAGTTCCAGGAACCATTGTTGCGTAAATGGATATCATTCAGAACAGTGGaataat > 1:3106890/2‑58 (MQ=17) aaaTAGTTCCAGGAACCATTGTTGCGTAAATGGATATCATTCAGAACAGTGGaataat > 1:1187370/2‑58 (MQ=17) aaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGa > 1:28939/1‑52 (MQ=255) | GTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGG > minE/359349‑359482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |