Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 377,111 A→C 12.8% intergenic (‑25/‑86) fepD ← / → ybdA iron‑enterobactin transporter subunit/predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE377,1110AC12.8% 161.5 / 20.8 70intergenic (‑25/‑86)fepD/ybdAiron‑enterobactin transporter subunit/predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (27/34);  new base C (6/3);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTG  >  minE/377044‑377180
                                                                   |                                                                     
cGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaattaat                                                                     >  1:2157397/1‑70 (MQ=255)
cGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaattaat                                                                     >  1:1942898/1‑70 (MQ=255)
 gggCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAattaatta                                                                   <  1:86053/71‑1 (MQ=255)
  ggCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttat                                                                  >  1:260524/1‑71 (MQ=255)
   gCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatc                                                                 >  1:2714233/1‑71 (MQ=255)
     aCGGCAATGGCGCGTGTCACGGCAACAGAACCAGTCATACGAACTTCCATGATAATGAAATTAAttatcgt                                                               >  1:3112033/1‑71 (MQ=255)
         cAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAattaatta                                                                   >  1:2334295/1‑63 (MQ=255)
          aaTGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                          <  1:1020459/71‑1 (MQ=255)
          aaTGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                          <  1:407331/71‑1 (MQ=255)
            tGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                        <  1:3363074/71‑1 (MQ=255)
             ggCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATc                                                       >  1:1962610/1‑71 (MQ=255)
             ggCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATc                                                       >  1:1958223/1‑71 (MQ=255)
              gcgcGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcaga                                                        >  1:2840828/1‑66 (MQ=255)
                gcgTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTa                                                    <  1:1282427/71‑1 (MQ=255)
                 cgTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTAt                                                   <  1:71599/71‑1 (MQ=255)
                     tCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTgg                                               <  1:1618884/71‑1 (MQ=255)
                       aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                        <  1:2282973/60‑1 (MQ=255)
                       aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                        <  1:2068965/60‑1 (MQ=255)
                       aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTAt                                                   <  1:2735353/65‑1 (MQ=255)
                       aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              >  1:3170685/1‑70 (MQ=255)
                           cAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         <  1:535955/71‑1 (MQ=255)
                            aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGtt                                        <  1:3209827/71‑1 (MQ=255)
                            aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:3224774/1‑70 (MQ=255)
                            aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:288609/1‑70 (MQ=255)
                            aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:1157318/1‑70 (MQ=255)
                             aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:1575665/1‑69 (MQ=255)
                             aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:3542214/1‑69 (MQ=255)
                             aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                         >  1:1536296/1‑69 (MQ=255)
                              cAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgt                                                               <  1:106851/46‑1 (MQ=255)
                               aGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGc                                     <  1:3246406/71‑1 (MQ=255)
                               aGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGc                                     <  1:2761244/71‑1 (MQ=255)
                                gAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCa                                    <  1:101615/71‑1 (MQ=255)
                                gAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCa                                    <  1:3551172/71‑1 (MQ=255)
                                 aaCCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                                   >  1:750521/1‑71 (MQ=255)
                                 aaCCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                                   >  1:3077840/1‑71 (MQ=255)
                                   ccAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                          <  1:510616/46‑1 (MQ=255)
                                   ccAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                          <  1:2240170/46‑1 (MQ=255)
                                      gACATACGAACTTCCATGATAATGAAATTAATTATAGTTATCGATCTTATTTGGATATGTTAGCAtgtg                                >  1:1581571/1‑69 (MQ=255)
                                       aCATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              <  1:2695204/54‑1 (MQ=255)
                                       aCATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              <  1:312530/54‑1 (MQ=255)
                                       aCATACGAACTTCCATGATAATGAAATTAATTATCGTTTCGATCTTATTTGGa                                              <  1:314989/53‑1 (MQ=255)
                                           aCGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCtt                                                     <  1:2304308/43‑1 (MQ=255)
                                           aCGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCtt                                                     <  1:117190/43‑1 (MQ=255)
                                           aCGAACTTCCATGACAATGAAATTAATTATCGTTATCGATCtt                                                     <  1:1981079/43‑1 (MQ=255)
                                            cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              <  1:255102/49‑1 (MQ=255)
                                            cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              <  1:1079328/49‑1 (MQ=255)
                                            cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                              <  1:1029691/49‑1 (MQ=255)
                                            cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                          >  1:3660957/1‑69 (MQ=255)
                                            cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                          >  1:1027629/1‑69 (MQ=255)
                                             gAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                          >  1:3152176/1‑68 (MQ=255)
                                                cTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAAt                    <  1:2137943/71‑1 (MQ=255)
                                                  tCCATGATATTGATATTAATTATCGTTATCGATCtt                                                     <  1:2638157/36‑1 (MQ=37)
                                                   ccATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATa                   <  1:1922845/69‑1 (MQ=255)
                                                   ccATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAgg                 >  1:3277673/1‑71 (MQ=255)
                                                    cATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAgg                 <  1:3021942/70‑1 (MQ=255)
                                                    aacgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                          <  1:2679491/58‑1 (MQ=37)
                                                     acgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                                   >  1:977289/3‑51 (MQ=25)
                                                     acgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                                   >  1:1251318/3‑51 (MQ=25)
                                                      tGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt              >  1:3149905/1‑71 (MQ=255)
                                                      tGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt              >  1:1915890/1‑71 (MQ=255)
                                                       gATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATg                                          <  1:643583/42‑1 (MQ=25)
                                                       gATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATg                                          <  1:1607141/42‑1 (MQ=25)
                                                       gATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAtt             >  1:986834/1‑71 (MQ=255)
                                                       gATAATGAAATTAATTATCGTTATCGATATTATTTGGATATGTTAGc                                     >  1:1957861/1‑47 (MQ=255)
                                                         taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                        >  1:2008541/1‑42 (MQ=25)
                                                         taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                        >  1:255457/1‑42 (MQ=25)
                                                         taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                        >  1:144464/1‑42 (MQ=25)
                                                         taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                        >  1:1089039/1‑42 (MQ=25)
                                                         tAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTa           <  1:534874/71‑1 (MQ=255)
                                                         tAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTa           <  1:818938/71‑1 (MQ=255)
                                                         tAATGAAATTAATTATAGTTATCGATCTTATTTGGATATGTTAGCAt                                   >  1:3628334/1‑47 (MQ=255)
                                                           aTGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAATAATAGGTATTTaa          <  1:2398993/70‑1 (MQ=255)
                                                            tGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt              >  1:801841/1‑65 (MQ=255)
                                                            tGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt              >  1:764446/1‑65 (MQ=255)
                                                                attaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAata      <  1:3717363/69‑1 (MQ=255)
                                                                attaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAata      <  1:1289861/69‑1 (MQ=255)
                                                                 ttaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAata      <  1:3703250/68‑1 (MQ=255)
                                                                 ttaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATAttt   >  1:1419068/1‑71 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAg                  >  1:2802164/1‑54 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAg                  >  1:2921621/1‑54 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAg                  >  1:66148/1‑54 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAg                  >  1:1469584/1‑54 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAg                  >  1:1678707/1‑54 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTg  <  1:758167/70‑1 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTg  <  1:1316689/70‑1 (MQ=255)
                                                                   aattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTg  <  1:2250581/70‑1 (MQ=255)
                                                                   |                                                                     
CGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATATTTG  >  minE/377044‑377180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: