| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 40,322 | C→T | 5.7% | P81L (CCG→CTG) | fixA → | predicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 40,322 | 0 | C | T | 5.7% | 339.4 / 6.7 | 104 | P81L (CCG→CTG) | fixA | predicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (46/52); new base T (3/3); total (49/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
ACAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT > minE/40252‑40391 | aCAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGccc > 1:2795670/1‑71 (MQ=255) cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg < 1:1117365/71‑1 (MQ=255) cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg > 1:1659519/1‑71 (MQ=255) cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg > 1:416671/1‑71 (MQ=255) cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg > 1:174239/1‑71 (MQ=255) cAGCCTTAAGTGGGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg < 1:682260/71‑1 (MQ=255) gCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa < 1:597107/71‑1 (MQ=255) ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt > 1:2736683/1‑71 (MQ=255) ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt < 1:1418417/71‑1 (MQ=255) tAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:2736496/1‑71 (MQ=255) aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa > 1:1915158/1‑66 (MQ=255) aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGa < 1:165923/69‑1 (MQ=255) aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGa < 1:2442613/69‑1 (MQ=255) aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAAc > 1:2301033/1‑71 (MQ=255) aGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACt < 1:2134842/71‑1 (MQ=255) gtgtGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACt > 1:2177700/1‑70 (MQ=255) tgtgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGa < 1:1798842/71‑1 (MQ=255) tgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGAtt > 1:487716/1‑71 (MQ=255) tgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGAtt > 1:3544786/1‑71 (MQ=255) ggCGGTAAAGCCCTTACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg < 1:617844/70‑1 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:3663493/71‑1 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg < 1:2337582/70‑1 (MQ=255) gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt < 1:1204609/67‑1 (MQ=255) gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt < 1:914451/67‑1 (MQ=255) gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt < 1:3327001/67‑1 (MQ=255) gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg > 1:3593964/1‑71 (MQ=255) tAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAattgattg < 1:8051/65‑1 (MQ=255) tAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg < 1:2148054/65‑1 (MQ=255) aaaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat > 1:1443036/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt > 1:289964/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:1823803/70‑1 (MQ=255) aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa < 1:181808/51‑1 (MQ=255) aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattg < 1:3545868/71‑1 (MQ=255) aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt > 1:2130979/1‑70 (MQ=255) gCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga < 1:1116821/71‑1 (MQ=255) tGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat < 1:1704763/68‑1 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg > 1:3041212/1‑59 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg > 1:1032475/1‑59 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg > 1:1161172/1‑59 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt > 1:3105361/1‑46 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:3620101/1‑70 (MQ=255) gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:1741470/1‑70 (MQ=255) ccAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc < 1:1449023/68‑1 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg < 1:1044255/53‑1 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg < 1:3735032/53‑1 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg < 1:3060515/53‑1 (MQ=255) cGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc > 1:3196553/1‑70 (MQ=255) gCCAAAGGGCGTGAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc > 1:728921/1‑69 (MQ=255) gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTgtggt > 1:464765/1‑53 (MQ=255) gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc > 1:2531707/1‑69 (MQ=255) gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc > 1:903135/1‑69 (MQ=255) ccAAAGGGCGTAAAGATGTTCTATCGCGCGGCCCGGATGAACTGATtg > 1:2461243/1‑48 (MQ=255) ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg > 1:2725510/1‑48 (MQ=255) ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg > 1:2853047/1‑48 (MQ=255) cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATtgatga > 1:1491540/1‑60 (MQ=255) cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATtgatga > 1:2944526/1‑60 (MQ=255) cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg > 1:2238661/1‑70 (MQ=255) aaaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGtt > 1:3566322/1‑65 (MQ=255) aaaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGtt > 1:3581954/1‑65 (MQ=255) aaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:923493/46‑1 (MQ=255) aGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAgg < 1:3677618/71‑1 (MQ=255) gggCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGc < 1:3643301/71‑1 (MQ=255) gggCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGc < 1:1214239/71‑1 (MQ=255) cGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACt < 1:2240839/71‑1 (MQ=255) cGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACt < 1:386949/71‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTgtggt < 1:1164383/42‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc < 1:2948967/71‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc < 1:3096832/71‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCACGGATGAACTGATTgtggt < 1:845930/42‑1 (MQ=255) aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg < 1:247612/60‑1 (MQ=255) aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg < 1:3057863/60‑1 (MQ=255) aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCa < 1:3308049/62‑1 (MQ=255) aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGcc > 1:1258197/1‑71 (MQ=255) aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGcc > 1:1518105/1‑71 (MQ=255) aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat > 1:773324/1‑47 (MQ=255) aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat > 1:146351/1‑47 (MQ=255) aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat > 1:2054567/1‑47 (MQ=255) aGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCg < 1:3626871/70‑1 (MQ=255) gATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:185235/41‑1 (MQ=255) gATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGca < 1:2317170/71‑1 (MQ=255) aTGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGt < 1:2611095/51‑1 (MQ=255) tgtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaa > 1:476312/1‑70 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCa < 1:2401416/47‑1 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCa < 1:3345284/47‑1 (MQ=255) cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc < 1:1308624/48‑1 (MQ=255) cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAAc > 1:1002405/1‑71 (MQ=255) cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAAc < 1:3001104/71‑1 (MQ=255) tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCa < 1:3312378/52‑1 (MQ=255) tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACgg < 1:3525639/70‑1 (MQ=255) tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGc < 1:2313260/71‑1 (MQ=255) tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGc < 1:1667585/71‑1 (MQ=255) gcgcgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGc < 1:1790498/47‑1 (MQ=255) cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg < 1:1317377/71‑1 (MQ=255) cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg < 1:2758419/71‑1 (MQ=255) cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg < 1:2205278/71‑1 (MQ=255) cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGa < 1:3490668/65‑1 (MQ=255) cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc < 1:3495318/71‑1 (MQ=255) cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc < 1:2403885/71‑1 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt > 1:1415494/1‑71 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt < 1:1644501/71‑1 (MQ=255) | ACAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT > minE/40252‑40391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |