| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 62,679 | A→G | 71.4% | F260F (TTT→TTC) | polB ← | DNA polymerase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 62,679 | 0 | A | G | 71.4% | 62.3 / 28.4 | 42 | F260F (TTT→TTC) | polB | DNA polymerase II |
| Reads supporting (aligned to +/- strand): ref base A (5/7); new base G (19/11); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT > minE/62613‑62746 | tttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGc > 1:2690478/1‑71 (MQ=255)tttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGc > 1:2394792/1‑71 (MQ=255) gcCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCg < 1:2398823/66‑1 (MQ=255) gcCTCGATACCGTAGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCt < 1:2944209/70‑1 (MQ=255) ccTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTcg < 1:23099/71‑1 (MQ=255) cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCgcgc > 1:1588657/1‑71 (MQ=255) gATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCgcgc > 1:2394126/1‑70 (MQ=255) aCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAg > 1:343245/1‑55 (MQ=255) aCCGGCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGcc < 1:1962456/57‑1 (MQ=255) tCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg > 1:1429094/1‑71 (MQ=255) cGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTcc > 1:2585803/1‑68 (MQ=255) aTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCa > 1:1042706/1‑62 (MQ=255) aTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCa > 1:480745/1‑62 (MQ=255) tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTc > 1:946959/1‑70 (MQ=255) tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCg > 1:1610636/1‑71 (MQ=255) tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCg > 1:1435419/1‑71 (MQ=255) aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg < 1:1749754/65‑1 (MQ=255) aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg < 1:3452304/65‑1 (MQ=255) aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg < 1:2104381/65‑1 (MQ=255) tAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtat > 1:729526/1‑71 (MQ=255) gCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtatt < 1:623827/70‑1 (MQ=255) cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTg < 1:300852/40‑1 (MQ=255) ttAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc > 1:3683902/1‑70 (MQ=255) tAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc < 1:2535342/69‑1 (MQ=255) gggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACg > 1:2883401/1‑70 (MQ=255) ggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTcc > 1:2814008/1‑43 (MQ=255) cAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGc < 1:3224207/71‑1 (MQ=255) aaaaGACGCCGTTTTTGAAGCCGTGCTCGCGCCACt > 1:117696/1‑36 (MQ=255) aGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGa > 1:3140727/1‑71 (MQ=255) cGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCgg < 1:3263583/64‑1 (MQ=255) cGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCgg < 1:1032486/64‑1 (MQ=255) gCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACgg > 1:360274/1‑70 (MQ=255) gTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtattat > 1:3548458/1‑41 (MQ=255) gTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtattat > 1:1436777/1‑41 (MQ=255) tttttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACg > 1:257212/1‑71 (MQ=255) tttttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACg > 1:2533294/1‑71 (MQ=255) tttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGc > 1:366476/1‑70 (MQ=255) ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg < 1:1273132/53‑1 (MQ=255) ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg < 1:1362190/53‑1 (MQ=255) ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg < 1:3460936/53‑1 (MQ=255) ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg < 1:945836/53‑1 (MQ=255) ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCt < 1:1900921/70‑1 (MQ=255) | TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT > minE/62613‑62746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |