Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,215	T→G	5.8%	E680A (GAA→GCA) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,215	0	T	G	5.8%	232.4 / 8.2	86	E680A (GAA→GCA) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (36/45);  new base G (2/3);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGC  >  minE/608145‑608282                                                                       |                                                                    accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACtt                                                                     <  1:3205139/71‑1 (MQ=255)  ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    >  1:831423/1‑71 (MQ=255)  ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    <  1:599943/71‑1 (MQ=255)  ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    <  1:1910382/71‑1 (MQ=255)  ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    <  1:1914503/71‑1 (MQ=255)   caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg                                                                   <  1:2452448/71‑1 (MQ=255)      cTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtcg                                                                >  1:1584842/1‑71 (MQ=255)         cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                               <  1:158074/69‑1 (MQ=255)         cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                               <  1:3281764/69‑1 (MQ=255)         cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                                             >  1:1257412/1‑71 (MQ=255)          gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                                            <  1:2778301/71‑1 (MQ=255)           atggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    <  1:547807/62‑1 (MQ=255)             ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                         >  1:978465/1‑71 (MQ=255)             ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                         >  1:1084660/1‑71 (MQ=255)             ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                         >  1:1056648/1‑71 (MQ=255)              gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTAGTCTTTAcc                                                        <  1:1333530/71‑1 (MQ=255)               atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                                       <  1:3641242/71‑1 (MQ=255)               atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                                       <  1:1049229/71‑1 (MQ=255)                  cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                                                    <  1:2006578/71‑1 (MQ=255)                   ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                                   >  1:3317690/1‑69 (MQ=255)                   ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                                   >  1:555346/1‑69 (MQ=255)                       tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                                      >  1:1810574/1‑64 (MQ=255)                       tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                                      >  1:2123364/1‑64 (MQ=255)                       ctgtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                                <  1:1079716/68‑1 (MQ=255)                          tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                                <  1:1732766/67‑1 (MQ=255)                          tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                                <  1:1129711/67‑1 (MQ=255)                          tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                                <  1:2592002/67‑1 (MQ=255)                          tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                            <  1:1470584/71‑1 (MQ=255)                            gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                    <  1:2564473/45‑1 (MQ=255)                            gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                            <  1:81576/69‑1 (MQ=255)                              aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACGCAGTGCGTTCGGAAc                                         >  1:3145403/1‑70 (MQ=255)                              aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                            <  1:2212706/67‑1 (MQ=255)                              aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                            <  1:3243649/67‑1 (MQ=255)                                  aCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                                              <  1:2868811/45‑1 (MQ=255)                                    catccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc                                         <  1:2684017/64‑1 (MQ=255)                                     atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                                  >  1:2197010/1‑70 (MQ=255)                                     atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                                  >  1:2396999/1‑70 (MQ=255)                                     atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                                 <  1:1198443/71‑1 (MQ=255)                                     atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                                 <  1:983901/71‑1 (MQ=255)                                     atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                                 <  1:2909350/71‑1 (MQ=255)                                       ccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAg                               >  1:2451130/1‑71 (MQ=255)                                        catcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAgg                              >  1:3194138/1‑71 (MQ=255)                                           cAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg                            >  1:2079794/1‑70 (MQ=255)                                            aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                           <  1:2219934/70‑1 (MQ=255)                                            aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                           <  1:541844/70‑1 (MQ=255)                                            aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                           <  1:1355717/70‑1 (MQ=255)                                             gACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                         <  1:189502/71‑1 (MQ=255)                                               ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                             >  1:179306/1‑49 (MQ=255)                                               ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                             >  1:2847790/1‑49 (MQ=255)                                               ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                             >  1:2560959/1‑49 (MQ=255)                                               ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                         >  1:470641/1‑69 (MQ=255)                                                cagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGGACGATAGAGAAGGTGTAg                         >  1:341976/1‑68 (MQ=255)                                                 agccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                         >  1:3269415/1‑67 (MQ=255)                                                 agccagGTTGGTCTTACGAACTTCGTCGTCTATACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                          >  1:2142812/1‑66 (MQ=255)                                                  gccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga                     >  1:2956301/1‑70 (MQ=255)                                                   ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                                  <  1:2029097/56‑1 (MQ=255)                                                   ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATa                   <  1:3022282/71‑1 (MQ=255)                                                    cagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAc                  >  1:2464540/1‑71 (MQ=255)                                                     agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga                     <  1:1493229/67‑1 (MQ=255)                                                     agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc                 <  1:601451/71‑1 (MQ=255)                                                     agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc                 <  1:1577235/71‑1 (MQ=255)                                                      ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa                >  1:1274257/1‑71 (MQ=255)                                                      ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa                <  1:2778570/71‑1 (MQ=255)                                                       gTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt               <  1:632466/71‑1 (MQ=255)                                                       gTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt               <  1:2998289/71‑1 (MQ=255)                                                        ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc              <  1:672893/71‑1 (MQ=255)                                                        ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc              <  1:3163479/71‑1 (MQ=255)                                                        ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc              <  1:2991555/71‑1 (MQ=255)                                                        ttGGTCTTACGAACTTGGTCGTCTTTACCCAGTGCTTTCGGAACGATAGAGAACGTGTAGGAGATACCa                <  1:3379116/69‑1 (MQ=255)                                                        ttGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATc              >  1:3214630/1‑71 (MQ=255)                                                        ttGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATc              >  1:1002129/1‑71 (MQ=255)                                                         tGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt             >  1:3175971/1‑71 (MQ=255)                                                          ggTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt             <  1:3048909/70‑1 (MQ=255)                                                           gTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt            <  1:3229215/70‑1 (MQ=255)                                                            tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATaga                                   <  1:225981/46‑1 (MQ=255)                                                            tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt            <  1:2306891/69‑1 (MQ=255)                                                            tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt             <  1:343722/68‑1 (MQ=255)                                                             cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                    <  1:989949/44‑1 (MQ=255)                                                             cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                    <  1:1673309/44‑1 (MQ=255)                                                             cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                    <  1:3274462/44‑1 (MQ=255)                                                             cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                    <  1:2567041/44‑1 (MQ=255)                                                              ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                          >  1:3565558/1‑53 (MQ=255)                                                              ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                          >  1:200017/1‑53 (MQ=255)                                                              ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGga                       >  1:215671/1‑56 (MQ=255)                                                              ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGc        >  1:2327995/1‑71 (MQ=255)                                                                aCGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCg       >  1:3613149/1‑70 (MQ=255)                                                                 cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt      >  1:1394865/1‑70 (MQ=255)                                                                 cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt      >  1:2259443/1‑70 (MQ=255)                                                                 cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt      <  1:3484541/70‑1 (MQ=255)                                                                 cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTa     <  1:543070/71‑1 (MQ=255)                                                                    aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAg   >  1:613120/1‑70 (MQ=255)                                                                    aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAg   >  1:719779/1‑70 (MQ=255)                                                                    aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAg   >  1:913473/1‑70 (MQ=255)                                                                    aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAg   >  1:3429852/1‑70 (MQ=255)                                                                    aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGc  <  1:3072800/71‑1 (MQ=255)                                                                       |                                                                    ACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGC  >  minE/608145‑608282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑