| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 723,178 | T→C | 12.0% | T149A (ACC→GCC) | rne ← | fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 723,178 | 0 | T | C | 12.0% | 131.4 / 9.9 | 50 | T149A (ACC→GCC) | rne | fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Reads supporting (aligned to +/- strand): ref base T (22/22); new base C (3/3); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAG > minE/723109‑723248 | cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTa < 1:2126240/71‑1 (MQ=255) aGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAc > 1:3419571/1‑71 (MQ=255) tGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcg < 1:2024618/71‑1 (MQ=255) aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGcc < 1:1961621/68‑1 (MQ=255) tAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCg < 1:2200781/71‑1 (MQ=255) aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt < 1:809831/71‑1 (MQ=255) gCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc < 1:33670/61‑1 (MQ=255) tGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTa > 1:1871809/1‑47 (MQ=255) gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGc > 1:2943488/1‑68 (MQ=255) ccTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga < 1:3365211/71‑1 (MQ=255) ccTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgag > 1:2096029/1‑70 (MQ=255) cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa < 1:3097443/71‑1 (MQ=255) cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa < 1:2506882/71‑1 (MQ=255) cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa < 1:2131506/71‑1 (MQ=255) ttCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTc < 1:426720/57‑1 (MQ=255) tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa < 1:2078974/58‑1 (MQ=255) ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaaa < 1:1655508/69‑1 (MQ=255) ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg > 1:3104243/1‑71 (MQ=255) ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGaaa < 1:1351433/69‑1 (MQ=255) cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATa > 1:1718521/1‑58 (MQ=255) gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCgaga > 1:2672832/1‑61 (MQ=255) ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga < 1:1710693/60‑1 (MQ=255) ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg < 1:1487525/71‑1 (MQ=255) ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg < 1:1502009/71‑1 (MQ=255) cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAAt > 1:1078752/1‑61 (MQ=255) cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg > 1:506822/1‑71 (MQ=255) cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc < 1:2717541/70‑1 (MQ=255) aaGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa > 1:640201/1‑47 (MQ=255) aaGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa > 1:390135/1‑47 (MQ=255) aaGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgc > 1:2257428/1‑71 (MQ=255) aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcg < 1:2319902/71‑1 (MQ=255) aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg > 1:3045707/1‑69 (MQ=255) aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg > 1:261677/1‑69 (MQ=255) ggCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgc < 1:291688/71‑1 (MQ=255) cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCgg > 1:3273684/1‑71 (MQ=255) cTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATa > 1:410491/1‑45 (MQ=255) gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc > 1:695376/1‑62 (MQ=255) gCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATa < 1:1520116/42‑1 (MQ=255) cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg > 1:2430196/1‑52 (MQ=255) cttcttTTAATTCGGTACGGTCGTCGCCTTCGATACg > 1:140194/1‑37 (MQ=255) cttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCgg > 1:3565498/1‑70 (MQ=255) cttcttTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGaa < 1:3358839/44‑1 (MQ=255) tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGgttgtag > 1:1408868/1‑64 (MQ=255) tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt > 1:3218098/1‑71 (MQ=255) cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt < 1:1705933/70‑1 (MQ=255) ttttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt > 1:3106038/1‑69 (MQ=255) ttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAg > 1:275913/1‑70 (MQ=255) aTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAcc < 1:401396/71‑1 (MQ=255) tCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGcc > 1:1558079/1‑38 (MQ=255) gTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTAGGCATCAGAACCAGAt < 1:2664584/70‑1 (MQ=255) tACGGTAGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAg > 1:1679804/1‑71 (MQ=255) | CAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAG > minE/723109‑723248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |