| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 753,855 | T→C | 51.1% | N189S (AAC→AGC) | ycfT ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 753,855 | 0 | T | C | 51.1% | 2.2 / 136.9 | 92 | N189S (AAC→AGC) | ycfT | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (26/19); new base C (26/21); total (52/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
ACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAG > minE/753788‑753922 | acaGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCa < 1:1041803/71‑1 (MQ=255)acaGGTAATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCa > 1:2767517/1‑71 (MQ=255) aGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCAt < 1:1944292/70‑1 (MQ=255) attattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCcca > 1:2316614/1‑71 (MQ=255) tattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCccac > 1:2237335/1‑70 (MQ=255) attGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCccacca > 1:3291180/1‑71 (MQ=255) ttgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCcaccac < 1:982639/71‑1 (MQ=255) ttgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCcaccac < 1:944791/71‑1 (MQ=255) ttgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCcaccac < 1:695000/71‑1 (MQ=255) ttgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCccac < 1:2544512/68‑1 (MQ=255) tgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCcaccac < 1:2270086/70‑1 (MQ=255) gttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGt > 1:1641178/1‑55 (MQ=255) gttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGt > 1:2968385/1‑55 (MQ=255) ttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa < 1:1540727/57‑1 (MQ=255) ttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa < 1:559461/57‑1 (MQ=255) ttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa < 1:487604/57‑1 (MQ=255) ttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa < 1:742374/57‑1 (MQ=255) ttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa < 1:2600336/57‑1 (MQ=255) gcgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGt > 1:3671398/1‑71 (MQ=255) cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGt < 1:765682/70‑1 (MQ=255) cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTAATTCCCCACCACGGCGt < 1:329997/70‑1 (MQ=255) ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg < 1:3310761/67‑1 (MQ=255) ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg > 1:1661210/1‑69 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTc > 1:1242477/1‑50 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCa > 1:637928/1‑51 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCa > 1:3302958/1‑51 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTc > 1:1197400/1‑54 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTc > 1:1652768/1‑54 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGa < 1:2348655/71‑1 (MQ=255) cAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACAATGCTCATTc > 1:1214273/1‑54 (MQ=255) aaaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTc > 1:1400853/1‑49 (MQ=255) aaaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTgg > 1:3391149/1‑68 (MQ=255) aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAAc > 1:3369017/1‑71 (MQ=255) aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc > 1:1959347/1‑71 (MQ=255) tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcccc > 1:3496980/1‑50 (MQ=255) tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcccc > 1:1867928/1‑50 (MQ=255) tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcccc > 1:2376950/1‑50 (MQ=255) tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcccc > 1:1778318/1‑50 (MQ=255) tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGT‑GGAACGAAAt < 1:2719873/70‑1 (MQ=255) gcgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt > 1:642673/1‑70 (MQ=255) cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:3729189/70‑1 (MQ=255) cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:276030/70‑1 (MQ=255) cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:1589951/70‑1 (MQ=255) gcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTaa < 1:194458/71‑1 (MQ=255) gcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg < 1:1180650/58‑1 (MQ=255) gcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg < 1:502807/58‑1 (MQ=255) gcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg < 1:2314104/58‑1 (MQ=255) aGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc > 1:3452068/1‑71 (MQ=255) gCTGTAATATGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGa < 1:1992204/59‑1 (MQ=255) cTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCca > 1:1632074/1‑70 (MQ=255) tGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGa < 1:2822333/57‑1 (MQ=255) gTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca > 1:2180941/1‑70 (MQ=255) gTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCacac > 1:1916046/1‑71 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:1606184/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:62177/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:1610545/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:556250/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:3180000/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTccc > 1:322812/1‑36 (MQ=255) taataaGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTa < 1:2468057/60‑1 (MQ=255) aataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc > 1:3089096/1‑64 (MQ=255) aataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc > 1:3379981/1‑64 (MQ=255) aataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACt > 1:2397562/1‑70 (MQ=255) ataaGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTca < 1:631295/71‑1 (MQ=255) aaGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:3167667/1‑70 (MQ=255) aGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc < 1:756484/60‑1 (MQ=255) ggCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag > 1:2131973/1‑71 (MQ=255) ggCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag < 1:2440447/71‑1 (MQ=255) ggCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt > 1:672135/1‑52 (MQ=255) gCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGt < 1:1583676/40‑1 (MQ=255) gCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGt < 1:2836533/40‑1 (MQ=255) gCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGt < 1:1988138/40‑1 (MQ=255) gCAAATTGCGGATCACACTGTTCATTCCCCAACACGGCGt < 1:2447146/40‑1 (MQ=255) aaTTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTac > 1:63429/1‑70 (MQ=255) aaTTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTac > 1:1522871/1‑70 (MQ=255) aTTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTaca > 1:2552764/1‑70 (MQ=255) aTTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTaca > 1:1998719/1‑70 (MQ=255) cGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaaaca < 1:726476/70‑1 (MQ=255) cGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaaaca < 1:3101919/70‑1 (MQ=255) cGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaaaca < 1:3449945/70‑1 (MQ=255) aTCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGc < 1:575554/71‑1 (MQ=255) tCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCg < 1:2516461/71‑1 (MQ=255) cacaCTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGt > 1:954116/1‑58 (MQ=255) cacaCTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGt > 1:3727278/1‑58 (MQ=255) cacTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca > 1:3426951/1‑47 (MQ=255) cacTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAACCAAGGCGaa > 1:3267836/1‑70 (MQ=255) cacTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGc < 1:2351409/67‑1 (MQ=255) cTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc > 1:1403424/1‑41 (MQ=255) cTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAg > 1:1407985/1‑71 (MQ=255) gTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:2826742/1‑48 (MQ=255) gTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:716846/1‑48 (MQ=255) gTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:1736386/1‑48 (MQ=255) gTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:1980223/1‑48 (MQ=255) gTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:456228/1‑48 (MQ=255) | ACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAG > minE/753788‑753922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |