| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 757,001 | G→T | 8.8% | A143S (GCG→TCG) | lolE → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 757,001 | 0 | G | T | 8.8% | 228.3 / 5.4 | 68 | A143S (GCG→TCG) | lolE | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/43); new base T (3/3); total (22/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTG > minE/756933‑757068 | cGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCg < 1:1760918/71‑1 (MQ=255) gCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCgg > 1:1015018/1‑71 (MQ=255) aCAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg < 1:3289084/71‑1 (MQ=255) aCAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg < 1:1833081/71‑1 (MQ=255) aGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAATCGGGCGaa < 1:84537/71‑1 (MQ=255) aGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa < 1:1018430/71‑1 (MQ=255) cAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAATCGGGCGaa < 1:3410259/66‑1 (MQ=255) gCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg < 1:180105/62‑1 (MQ=255) cGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAAt < 1:3684380/71‑1 (MQ=255) cGTCTGAGCGCATTACCCTAGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAAt < 1:933661/71‑1 (MQ=255) ggcTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg < 1:1562504/58‑1 (MQ=255) cTGAGCGCATTACCCTCGTTTGTACAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGc > 1:1899133/1‑64 (MQ=255) gcgcATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatcatc < 1:1234089/71‑1 (MQ=255) cgcATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCg < 1:3595031/71‑1 (MQ=255) gcATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCgg > 1:1248329/1‑71 (MQ=255) aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTTAAGCGGGCGAACAGCAAATTATCATCGGCa < 1:1797253/71‑1 (MQ=255) aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCg < 1:2167293/68‑1 (MQ=255) aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCa < 1:3299888/71‑1 (MQ=255) aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCa < 1:1167855/71‑1 (MQ=255) ttACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCgg < 1:1352648/46‑1 (MQ=255) ttACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCgg < 1:1758023/68‑1 (MQ=255) tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCa < 1:1070573/69‑1 (MQ=255) tCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCaaa > 1:633405/1‑52 (MQ=255) gTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCa < 1:2107283/48‑1 (MQ=255) gTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCa < 1:3467720/48‑1 (MQ=255) gTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTg < 1:3441740/70‑1 (MQ=255) ttGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa > 1:81232/1‑41 (MQ=255) ttGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa > 1:2857015/1‑41 (MQ=255) ttGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa > 1:133422/1‑41 (MQ=255) ttGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa > 1:1553046/1‑41 (MQ=255) ttGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatcatc < 1:3432838/56‑1 (MQ=255) tGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCg < 1:2378149/70‑1 (MQ=255) ccAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTat < 1:2526918/48‑1 (MQ=255) ccAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTat < 1:2587511/48‑1 (MQ=255) ggCGATGCGTGGCGCAATTTTAAATCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCt > 1:2393580/1‑71 (MQ=255) ggCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCt > 1:293226/1‑71 (MQ=255) ggCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCt > 1:1078709/1‑71 (MQ=255) cGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGGAAAGGCGTGGCGGATGCGCTGa < 1:878856/71‑1 (MQ=255) cGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCtca < 1:3004704/71‑3 (MQ=255) cGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGa < 1:528863/71‑1 (MQ=255) gATGCGTGGCGCAATTTTAAATCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGaa < 1:2865324/71‑1 (MQ=255) gATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGaa < 1:2333835/71‑1 (MQ=255) aTGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGc > 1:3340388/1‑53 (MQ=255) aTGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGaaa < 1:922169/71‑1 (MQ=255) tGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatca < 1:14307/41‑1 (MQ=255) gCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCaa < 1:951033/47‑1 (MQ=255) gCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCaa < 1:418431/47‑1 (MQ=255) gCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTgg < 1:2364850/55‑1 (MQ=255) gTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGa < 1:3057235/71‑1 (MQ=255) gcgcAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGa < 1:2685402/62‑1 (MQ=255) gcgcAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAg > 1:2935008/1‑70 (MQ=255) cAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAgg < 1:473636/71‑1 (MQ=255) aaTTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAggg > 1:3262136/1‑71 (MQ=255) aaTTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAggg < 1:3491116/71‑1 (MQ=255) aTTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGa < 1:246180/57‑1 (MQ=255) aTTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAgggg > 1:1420697/1‑71 (MQ=255) aTTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAgggg > 1:1391446/1‑71 (MQ=255) ttttAAATCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAgggg > 1:2825616/1‑70 (MQ=255) ttttAAATCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAgggg > 1:1926723/1‑70 (MQ=255) ttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTg > 1:2548789/1‑53 (MQ=255) ttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTg > 1:1970880/1‑53 (MQ=255) tAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGAt < 1:3302270/70‑1 (MQ=255) tAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGT‑GCGGATGCGCTGAAAGTGAAGCAGGGGGAtt < 1:541073/70‑1 (MQ=255) aaaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGAtt < 1:858024/70‑1 (MQ=255) aaaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTg > 1:2918983/1‑71 (MQ=255) aaaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTg > 1:3012563/1‑71 (MQ=255) aaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTg < 1:466265/70‑1 (MQ=255) aaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTg < 1:1473184/70‑1 (MQ=255) gCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTg < 1:1733238/68‑1 (MQ=255) | CGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTG > minE/756933‑757068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |