| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 93,391 | G→T | 5.2% | G125C (GGC→TGC) ‡ | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 93,391 | 0 | G | T | 5.2% | 393.1 / 3.7 | 114 | G125C (GGC→TGC) ‡ | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base G (47/61); new base T (2/4); total (49/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ATGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGA > minE/93322‑93461 | aTGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAgg < 1:3380934/71‑1 (MQ=255)aTGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAgg > 1:2717448/1‑71 (MQ=255)aTGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAgg > 1:2119931/1‑71 (MQ=255) tGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGc < 1:2881196/71‑1 (MQ=255) gCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGc < 1:1218424/70‑1 (MQ=255) gCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGc < 1:2969752/70‑1 (MQ=255) gTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTg > 1:1451052/1‑71 (MQ=255) tACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTg > 1:2957844/1‑70 (MQ=255) ggTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGt < 1:1477433/71‑1 (MQ=255) gTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTa < 1:1175150/71‑1 (MQ=255) gAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:2410822/1‑71 (MQ=255) gAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:213817/1‑71 (MQ=255) gAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:40640/1‑71 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGtt < 1:3036875/71‑1 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGtt < 1:1695193/71‑1 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGtt < 1:723925/71‑1 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGtt < 1:1397037/71‑1 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:2987721/1‑70 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:2511688/1‑70 (MQ=255) aaGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt > 1:687499/1‑70 (MQ=255) gAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTAcc < 1:3572976/71‑1 (MQ=255) aaaaaCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt < 1:2452216/66‑1 (MQ=255) aaaCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCggt < 1:295092/71‑3 (MQ=21) aaaCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCggt < 1:230413/71‑3 (MQ=21) aaaCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCggt < 1:172975/71‑3 (MQ=21) aaaCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCggt < 1:2775245/71‑3 (MQ=21) aCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGtaac < 1:1243325/71‑4 (MQ=255) aCCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTc < 1:1297624/69‑1 (MQ=255) cccTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAAc < 1:1650267/71‑1 (MQ=255) ccTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAAc < 1:1678077/70‑1 (MQ=255) cTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCg > 1:2840826/1‑64 (MQ=21) cTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAACTACGTGCACGCCTTTACCg > 1:152414/1‑64 (MQ=21) gACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTAc > 1:1495129/1‑60 (MQ=255) aCCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGAc < 1:331779/69‑1 (MQ=255) aCCGCAAAGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGt < 1:2476413/56‑1 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTa > 1:210448/1‑56 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTa > 1:2195694/1‑56 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTa > 1:2256060/1‑56 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTa > 1:380900/1‑56 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTAcc > 1:775852/1‑71 (MQ=255) cGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTAcc > 1:2467446/1‑71 (MQ=255) cAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACg > 1:3231135/1‑63 (MQ=255) cAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACg > 1:1776948/1‑63 (MQ=255) cAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACg > 1:658442/1‑63 (MQ=255) cAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTg < 1:3360839/71‑1 (MQ=255) cAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTg < 1:750505/71‑1 (MQ=255) aaCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCa < 1:2409100/45‑1 (MQ=255) aaCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCa < 1:3643017/45‑1 (MQ=255) aaCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCa < 1:3217261/45‑1 (MQ=255) aaCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTgg > 1:1835621/1‑71 (MQ=255) aCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgc < 1:1247164/71‑1 (MQ=255) aCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgc < 1:1500866/71‑1 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc < 1:2260362/71‑1 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc < 1:92963/71‑1 (MQ=255) ctgcctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAAc > 1:1626588/1‑57 (MQ=255) cctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAAc < 1:491967/70‑1 (MQ=255) cctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAAc < 1:656381/70‑1 (MQ=255) ctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGt < 1:2346840/71‑1 (MQ=255) ctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGt < 1:2802575/71‑1 (MQ=255) gcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGa > 1:2849347/1‑71 (MQ=255) cTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGAc < 1:3657697/71‑1 (MQ=255) cTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGAc < 1:2873814/71‑1 (MQ=255) cTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGAc < 1:2569047/71‑1 (MQ=255) aCCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGcc > 1:3642335/1‑71 (MQ=255) ccTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGcc < 1:2003626/70‑1 (MQ=255) ccTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCg < 1:1585018/71‑1 (MQ=255) ccTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCg < 1:3475404/71‑1 (MQ=255) ccTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCg < 1:1798176/71‑1 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTAGCGTCAACGACTACCTg > 1:2649134/1‑54 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGcc > 1:3709759/1‑69 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCg > 1:572529/1‑70 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCg > 1:1722504/1‑70 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGa < 1:1809848/71‑1 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGa < 1:303433/71‑1 (MQ=255) cTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGa < 1:2561230/71‑1 (MQ=255) gAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaa > 1:2649424/1‑70 (MQ=255) gAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaa > 1:1089072/1‑70 (MQ=255) gAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaa > 1:752969/1‑70 (MQ=255) gAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaa > 1:3035325/1‑70 (MQ=255) aaCGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaaaa < 1:3119978/71‑1 (MQ=255) aaCGCACTAACCGGTAAAGGCGT‑CACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaaa < 1:2933344/69‑1 (MQ=255) aCGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAaac < 1:1386094/71‑1 (MQ=255) aCTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAAcc < 1:3200255/71‑1 (MQ=255) aCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAaac < 1:1433589/63‑1 (MQ=255) aCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAaac < 1:500332/63‑1 (MQ=255) aCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTcc > 1:93062/1‑71 (MQ=255) aCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTcc > 1:2912696/1‑71 (MQ=255) ccGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCg < 1:2263508/71‑1 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAaacaa > 1:3489152/1‑61 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg < 1:1736961/71‑1 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg < 1:2531559/71‑1 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg > 1:1625837/1‑71 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg < 1:154378/71‑1 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg > 1:3318555/1‑71 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCAACGACTACATGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg > 1:577675/1‑71 (MQ=255) aaGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg < 1:2073850/68‑1 (MQ=255) aaGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTg < 1:2424753/68‑1 (MQ=255) aaGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGttt < 1:1512766/71‑1 (MQ=255) ggCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGa < 1:2824296/50‑1 (MQ=255) ggCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGa < 1:872024/71‑1 (MQ=255) | ATGCGTACCGGTGAAGGAAAAACCCTGACCGCAACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGA > minE/93322‑93461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |