| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 776,453 | A→T | 7.1% | intergenic (+825/+40) | icd → / ← minE | isocitrate dehydrogenase, specific for NADP+/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 776,453 | 0 | A | T | 7.1% | 162.1 / 4.1 | 56 | intergenic (+825/+40) | icd/minE | isocitrate dehydrogenase, specific for NADP+/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base A (28/24); new base T (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
AGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGG > minE/776385‑776521 | agTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt > 1:3474811/1‑70 (MQ=255) tCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc > 1:3260366/1‑71 (MQ=255) tCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc > 1:1916563/1‑71 (MQ=255) aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg > 1:3172501/1‑71 (MQ=255) aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg > 1:2012210/1‑71 (MQ=255) cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa > 1:3552542/1‑64 (MQ=255) cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa > 1:2507747/1‑64 (MQ=255) aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt < 1:548973/71‑1 (MQ=255) aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt < 1:2832013/71‑1 (MQ=255) aaTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATctct > 1:1460710/1‑64 (MQ=255) aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg > 1:2649312/1‑69 (MQ=255) tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGa < 1:897572/69‑1 (MQ=255) gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa > 1:3638431/1‑71 (MQ=255) ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt < 1:453763/71‑1 (MQ=255) gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa > 1:1756953/1‑68 (MQ=255) gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc < 1:1898275/71‑1 (MQ=255) cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTAACTCTGCCTTGAAGATAAATgc > 1:1001135/1‑70 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa > 1:1751126/1‑59 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa > 1:1396019/1‑59 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa > 1:3593590/1‑59 (MQ=255) tACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtt > 1:624095/1‑71 (MQ=255) cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg < 1:2647049/48‑1 (MQ=255) acaACGTGGATGAGAGGGATGCAAAACTCAAGGCAGAGTTATct < 1:3398427/44‑1 (MQ=25) acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATct < 1:2011866/44‑1 (MQ=38) acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:628945/1‑70 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt < 1:3080871/39‑1 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:25068/1‑69 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:3098370/1‑69 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa < 1:2438200/71‑1 (MQ=255) aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt < 1:2248623/55‑1 (MQ=255) aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg > 1:2950024/1‑71 (MQ=255) aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg < 1:69960/71‑1 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc < 1:1651166/70‑1 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc < 1:1646827/70‑1 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt < 1:189615/71‑1 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt > 1:1526558/1‑71 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt > 1:2871981/1‑71 (MQ=255) aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg > 1:1876172/1‑67 (MQ=255) aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt < 1:1864710/71‑1 (MQ=255) tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa > 1:1787073/1‑71 (MQ=255) gagaGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt > 1:1618567/1‑71 (MQ=255) agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt < 1:878240/70‑1 (MQ=255) ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa > 1:2795716/1‑70 (MQ=255) gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg < 1:854733/58‑1 (MQ=255) aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg < 1:373709/57‑1 (MQ=255) tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGctc > 1:2747042/1‑71 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:3161510/1‑49 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt < 1:2518680/71‑1 (MQ=255) aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt < 1:1927952/70‑1 (MQ=255) cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt < 1:2257086/71‑1 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt < 1:389740/69‑1 (MQ=255) cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa < 1:393042/71‑1 (MQ=255) cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa < 1:1937541/71‑1 (MQ=255) cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa < 1:3434511/71‑1 (MQ=255) agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc > 1:1790938/1‑66 (MQ=255) agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt > 1:2497095/1‑69 (MQ=255) agaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg < 1:2385945/71‑1 (MQ=255) agaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg < 1:20749/71‑1 (MQ=255) agaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg < 1:1394815/71‑1 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc < 1:1148983/50‑1 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc < 1:1262463/50‑1 (MQ=255) | AGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGG > minE/776385‑776521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |