| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 792,959 | T→C | 6.5% | I443V (ATT→GTT) | cvrA ← | predicted cation/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 792,959 | 0 | T | C | 6.5% | 270.9 / 8.0 | 92 | I443V (ATT→GTT) | cvrA | predicted cation/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (48/38); new base C (2/4); total (50/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
ACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGA > minE/792889‑793029 | acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt < 1:1622244/71‑1 (MQ=255)acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt < 1:2747909/71‑1 (MQ=255)acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt < 1:1890368/71‑1 (MQ=255)acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt < 1:3679739/71‑1 (MQ=255) caTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATa < 1:3680946/71‑1 (MQ=255) caTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATa > 1:155704/1‑71 (MQ=255) gCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg > 1:3169493/1‑71 (MQ=255) gCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg > 1:2240018/1‑71 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtc > 1:1712626/1‑71 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt > 1:1968074/1‑70 (MQ=255) tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc > 1:1065465/1‑71 (MQ=255) tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc > 1:1079260/1‑71 (MQ=255) tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc > 1:193429/1‑71 (MQ=255) tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc > 1:1336352/1‑71 (MQ=255) tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc > 1:232508/1‑71 (MQ=255) gcgcAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtct > 1:560536/1‑68 (MQ=255) cAGTCGGGTGCTGACGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCtttt > 1:119954/1‑70 (MQ=255) ggTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt < 1:3642306/62‑1 (MQ=255) ggTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt < 1:3184558/62‑1 (MQ=255) gTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg < 1:201795/71‑1 (MQ=255) gTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg < 1:2632840/71‑1 (MQ=255) tgctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGTCATATGc < 1:1492866/71‑1 (MQ=255) tgctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg > 1:1082112/1‑70 (MQ=255) gctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt < 1:2035396/59‑1 (MQ=255) gctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGAGTCTCtt < 1:3038828/59‑1 (MQ=255) tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtttt > 1:1602719/1‑59 (MQ=255) tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt > 1:848966/1‑58 (MQ=255) gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt > 1:851288/1‑71 (MQ=255) gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt > 1:2493095/1‑71 (MQ=255) gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt > 1:1873080/1‑71 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTctct < 1:3207917/54‑1 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTctct < 1:2417147/54‑1 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa < 1:2283003/69‑1 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc > 1:3073932/1‑71 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc < 1:3621053/71‑1 (MQ=255) ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc < 1:1938564/71‑1 (MQ=255) gTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt < 1:2848842/47‑1 (MQ=255) gTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt < 1:2516428/47‑1 (MQ=255) tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt < 1:2133838/46‑1 (MQ=255) tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg > 1:585649/1‑70 (MQ=255) tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg > 1:644120/1‑70 (MQ=255) aGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtct > 1:1209474/1‑47 (MQ=255) ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg < 1:62047/71‑1 (MQ=255) ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg > 1:220998/1‑71 (MQ=255) ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg > 1:698983/1‑71 (MQ=255) tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt > 1:3496105/1‑47 (MQ=255) tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt > 1:1444253/1‑47 (MQ=255) tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa > 1:1928597/1‑60 (MQ=255) tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGc > 1:2886290/1‑71 (MQ=255) tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGc > 1:1008415/1‑71 (MQ=255) aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGcc < 1:864595/70‑1 (MQ=255) aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg < 1:2834723/71‑1 (MQ=255) aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg < 1:1466947/71‑1 (MQ=255) gCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt < 1:1051737/42‑1 (MQ=255) gCAACTGGTTATCACGAAACAGTGCCGCAATACGCGGCTCTTTTGGCATATGCAAATCGCGCAGTGCcgcg < 1:3621203/71‑1 (MQ=255) cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc < 1:2567685/52‑1 (MQ=255) cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc < 1:2208956/52‑1 (MQ=255) cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc < 1:1993530/52‑1 (MQ=255) aaCTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGcc > 1:634550/1‑71 (MQ=255) aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtttt < 1:3402942/41‑1 (MQ=255) aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGt > 1:814650/1‑62 (MQ=255) aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGt > 1:2113095/1‑62 (MQ=255) aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGccc < 1:1673288/71‑1 (MQ=255) cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa < 1:1324449/71‑1 (MQ=255) cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa > 1:1486678/1‑71 (MQ=255) cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa < 1:3115980/71‑1 (MQ=255) cTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGc < 1:2554920/43‑1 (MQ=255) cTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGc < 1:1602248/43‑1 (MQ=255) ggTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg > 1:1950953/1‑60 (MQ=255) ggTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACg > 1:876201/1‑71 (MQ=255) ttATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcgc < 1:3477933/62‑1 (MQ=255) aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc < 1:3538933/52‑1 (MQ=255) aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc < 1:873221/52‑1 (MQ=255) aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc < 1:2199420/52‑1 (MQ=255) aTCACGAAACAGTGCCGCAATACGCGTCTCTTTT‑GCATATGCAAATCgcgc < 1:3388436/51‑1 (MQ=255) tCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCAcc < 1:2267553/70‑1 (MQ=255) tCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCAcc < 1:2119595/70‑1 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:2498561/1‑48 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:1485154/1‑48 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:923866/1‑48 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:3642296/1‑48 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:1897238/1‑48 (MQ=255) gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa > 1:1575955/1‑48 (MQ=255) aaaCAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt < 1:1877754/71‑1 (MQ=255) gTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg < 1:1079223/45‑1 (MQ=255) gTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg < 1:2707275/45‑1 (MQ=255) tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt > 1:3379971/1‑65 (MQ=255) tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt > 1:2397932/1‑65 (MQ=255) tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt > 1:1953057/1‑65 (MQ=255) tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt > 1:1297127/1‑65 (MQ=255) tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCAc > 1:1938431/1‑71 (MQ=255) cGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCa < 1:989668/71‑1 (MQ=255) aaTACGCGTCTCTTTTGGCATATGTAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAAtt > 1:1605743/1‑71 (MQ=255) aaTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAAt > 1:708624/1‑70 (MQ=255) aTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa > 1:3573543/1‑50 (MQ=255) aTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa > 1:1984093/1‑50 (MQ=255) tACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGa < 1:2739216/71‑1 (MQ=255) | ACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGA > minE/792889‑793029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |