| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 801,126 | A→G | 7.5% | M68T (ATG→ACG) | dhaH ← | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 801,126 | 0 | A | G | 7.5% | 347.2 / 14.7 | 119 | M68T (ATG→ACG) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base A (61/49); new base G (7/2); total (68/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 129 total aligned reads displayed.
GGCGATCTCGGGAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTACCAAT > minE/801059‑801196 | cgATCTCGGGAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCGTGAc > 1:2160866/1‑70 (MQ=255) gATCTCGGGAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCGTGAc > 1:3551180/1‑69 (MQ=255) gATCTCGGGAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGAc > 1:1159457/1‑69 (MQ=255) gggAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGa > 1:2871206/1‑62 (MQ=255) gggAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGTCCAGca < 1:1209561/68‑1 (MQ=255) gggAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGca < 1:3481863/68‑1 (MQ=255) gggAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGca < 1:1278213/68‑1 (MQ=255) cAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCgg < 1:1463193/71‑1 (MQ=255) cAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCgg < 1:1650071/71‑1 (MQ=255) aGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGc < 1:445978/71‑1 (MQ=255) gCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGa > 1:1582205/1‑54 (MQ=255) gCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:673941/1‑71 (MQ=255) gCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:685978/1‑71 (MQ=255) aaTTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCGTGACCAGCACATGGTCGGCATc < 1:2184228/71‑1 (MQ=255) aTTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGca < 1:674217/57‑1 (MQ=255) aTTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCAt < 1:1462151/69‑1 (MQ=255) aTTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCa < 1:1501541/71‑1 (MQ=255) ttCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCa < 1:1708141/70‑1 (MQ=255) ttCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAg < 1:3586419/71‑1 (MQ=255) ttCCAGCGAAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAg > 1:445638/1‑71 (MQ=255) ccAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCGTGACCAGCACATGGTCGGCATCAGc > 1:2179112/1‑70 (MQ=255) cAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCa < 1:887023/70‑1 (MQ=255) gTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTc < 1:1507776/71‑1 (MQ=255) tttCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACa < 1:2649150/65‑1 (MQ=255) tttCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACa < 1:3506391/65‑1 (MQ=255) tttCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCg < 1:2527558/71‑1 (MQ=255) tttCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCg < 1:106642/71‑1 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGa > 1:3468168/1‑65 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAtt > 1:1655723/1‑67 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAtt > 1:3165526/1‑67 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGa < 1:2859539/70‑1 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGAt > 1:1227506/1‑71 (MQ=255) tCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGAt > 1:381224/1‑71 (MQ=255) aGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATg < 1:78793/70‑1 (MQ=255) gCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATgg < 1:2220263/45‑1 (MQ=255) aCTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCt < 1:591740/71‑1 (MQ=255) aataatGCGCTACCCATATCCATCGTGACCAGCACATGGTCGGCATCAGCa > 1:3731530/1‑51 (MQ=255) aataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAAc > 1:2143206/1‑53 (MQ=255) aataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAtt > 1:1818074/1‑58 (MQ=255) aataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAtt > 1:3485376/1‑58 (MQ=255) aataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAt > 1:774653/1‑57 (MQ=255) aataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTcc < 1:2047712/69‑1 (MQ=255) aataatGCGCTACCCATATCCATAATGACCAGCACATGGTCGGCATCAGCAACAGAt > 1:2415612/1‑57 (MQ=255) ataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGAt > 1:2042317/1‑56 (MQ=255) ataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCt > 1:2044881/1‑66 (MQ=255) ataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCt > 1:3599945/1‑66 (MQ=255) ataatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCt > 1:1271943/1‑66 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCATATGGTCGGCa > 1:3410049/1‑43 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:1266126/1‑43 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:459933/1‑43 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:185309/1‑43 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCa > 1:2717473/1‑43 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCAt < 1:133033/44‑1 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCAt < 1:2591845/44‑1 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCAt < 1:3448858/44‑1 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCAt < 1:3249726/44‑1 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTc > 1:1142396/1‑57 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTc > 1:2420308/1‑57 (MQ=255) taatGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTc > 1:1070191/1‑57 (MQ=255) atGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACt < 1:2916908/71‑1 (MQ=255) tGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGc < 1:738155/45‑1 (MQ=255) tGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTcc < 1:3488176/64‑1 (MQ=255) tGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt < 1:1110628/66‑1 (MQ=255) tGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt < 1:1435284/66‑1 (MQ=255) gcgcTACCCATATCCATCGTGACCAGCACATGGTCg < 1:1469044/36‑1 (MQ=255) gcgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGc < 1:3022894/59‑1 (MQ=255) gcgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGc < 1:3646633/59‑1 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGc > 1:805568/1‑58 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt > 1:3543485/1‑64 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt > 1:1728766/1‑64 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt > 1:2117476/1‑64 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCAt > 1:683084/1‑64 (MQ=255) cgcTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTg < 1:3201915/71‑1 (MQ=255) tACCCATATCCATCGTGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACg > 1:1715883/1‑71 (MQ=255) cccATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACgg > 1:3695992/1‑70 (MQ=255) cccATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACgg > 1:3347478/1‑70 (MQ=255) cATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCAt > 1:2516019/1‑71 (MQ=255) cATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCAt < 1:424459/71‑1 (MQ=255) cATATCCATCATGACCAG‑‑CATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCg > 1:2252211/1‑71 (MQ=255) atatCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATc > 1:1887152/1‑71 (MQ=255) atatCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATc > 1:1678394/1‑71 (MQ=255) atatCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATc > 1:555215/1‑71 (MQ=255) tatCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCa < 1:2755465/54‑1 (MQ=255) tatCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCg > 1:1672378/1‑71 (MQ=255) atCCATCATGACCAGCACATGGTCGGCATCAGCAAc > 1:1052530/1‑36 (MQ=255) atCCATCATGACCAGCACATGGTCGGCATCAGCAAc > 1:2476923/1‑36 (MQ=255) atCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCgg > 1:3007656/1‑71 (MQ=255) atCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCgg > 1:3595371/1‑71 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATTGCCTCCATCACTTTGACGGCATCGGt < 1:3536697/71‑1 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACttt < 1:2725532/59‑1 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACttt < 1:2551865/59‑1 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGt < 1:3506395/71‑1 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGt < 1:3509256/71‑1 (MQ=255) tCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGt < 1:826802/71‑1 (MQ=255) ccATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTa > 1:151769/1‑71 (MQ=255) catcatGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTAc > 1:3042573/1‑71 (MQ=255) catcatGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCAATCACTTTGACGGCATCGGTAc > 1:1378928/1‑71 (MQ=255) tcatGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTAcc > 1:183895/1‑70 (MQ=255) atGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTACCAAt > 1:3233709/1‑71 (MQ=255) atGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCAACGGTACCAAt > 1:540175/1‑71 (MQ=255) | GGCGATCTCGGGAGCCAGCAATTCCAGCGCAGTTTCAGCACTCAATAATGCGCTACCCATATCCATCATGACCAGCACATGGTCGGCATCAGCAACAGATTCGATGGCCTCCATCACTTTGACGGCATCGGTACCAAT > minE/801059‑801196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |