Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,145 C→T 9.2% G1201G (GGC→GGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,1450CT 9.2% 230.0 / 10.9 76G1201G (GGC→GGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (38/31);  new base T (5/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

CCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGT  >  minE/835076‑835213
                                                                     |                                                                    
ccAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCt                                                                     <  1:343371/71‑1 (MQ=255)
ccAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCt                                                                     <  1:2950903/71‑1 (MQ=255)
  aaCAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGTCTAt                                                                   >  1:3616395/1‑71 (MQ=255)
  aaCAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTAt                                                                   >  1:2735250/1‑71 (MQ=255)
    cAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGc                                                                 <  1:2071713/71‑1 (MQ=255)
     aGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATg                                                                  >  1:271470/1‑69 (MQ=255)
         tggtggTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATc                                                            <  1:3510365/71‑1 (MQ=255)
           gtggtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCt                                                           <  1:2001988/70‑1 (MQ=255)
             tgtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTAt                                                                   <  1:3490522/59‑1 (MQ=255)
             ggtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCggcggc                                                                      >  1:2274878/1‑57 (MQ=255)
              gtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGc                                                        <  1:2795046/70‑1 (MQ=255)
                aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                     >  1:429898/1‑71 (MQ=255)
                aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                     >  1:2098608/1‑71 (MQ=255)
                aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                     >  1:1251060/1‑71 (MQ=255)
                aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                     >  1:3549546/1‑71 (MQ=255)
                 tCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATAc                                                    <  1:3102207/71‑1 (MQ=255)
                   cATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACgg                                                  <  1:3298958/71‑1 (MQ=255)
                     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATg                                                                  >  1:2715203/1‑53 (MQ=255)
                     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                >  1:3395370/1‑71 (MQ=255)
                     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                <  1:1131923/71‑1 (MQ=255)
                     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                <  1:1992560/71‑1 (MQ=255)
                      aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                               >  1:3048541/1‑71 (MQ=255)
                      aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                               >  1:2771878/1‑71 (MQ=255)
                       aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                               >  1:1526720/1‑70 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTg                                                          >  1:652701/1‑55 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTg                                                          >  1:905031/1‑55 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg                                                   >  1:2633978/1‑62 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg                                                   >  1:716266/1‑62 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                           >  1:1438096/1‑70 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                           >  1:1998371/1‑70 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                           >  1:314025/1‑70 (MQ=255)
                           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                           <  1:681022/70‑1 (MQ=255)
                            gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta                                         <  1:1524965/71‑1 (MQ=255)
                            gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta                                         <  1:566995/71‑1 (MQ=255)
                             tCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAactac                                        >  1:2711088/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACTGCTTTAActacta                                      <  1:1967627/71‑1 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt                                              <  1:1847513/63‑1 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt                                              <  1:3653860/63‑1 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                <  1:2014893/61‑1 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      >  1:2688957/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      >  1:491929/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      >  1:3344492/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      >  1:1197218/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      >  1:3427113/1‑71 (MQ=255)
                               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                      <  1:992946/71‑1 (MQ=255)
                                cccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTAt                                     <  1:2930574/71‑1 (MQ=255)
                                   gTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGc                                  <  1:26367/71‑1 (MQ=255)
                                        aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt                             <  1:3088007/71‑1 (MQ=255)
                                         cGCCGAAACGCACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa                            <  1:3688406/71‑1 (MQ=255)
                                         cGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa                            <  1:2263758/71‑1 (MQ=255)
                                          gCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAg                           <  1:3084032/71‑1 (MQ=255)
                                           ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg                          >  1:530452/1‑71 (MQ=255)
                                           ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg                          >  1:3466709/1‑71 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                    >  1:2168642/1‑60 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                    >  1:3650211/1‑60 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                    >  1:909239/1‑60 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                    >  1:2442846/1‑60 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt                         <  1:1185876/71‑1 (MQ=255)
                                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt                         <  1:109088/71‑1 (MQ=255)
                                             gAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCgg                          >  1:408821/1‑69 (MQ=255)
                                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGGACCGTCggat                        >  1:1525433/1‑68 (MQ=255)
                                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGt                             >  1:24556/1‑65 (MQ=255)
                                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc                       >  1:1549236/1‑71 (MQ=255)
                                               aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc                       >  1:2450242/1‑70 (MQ=255)
                                                aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt                      >  1:2220365/1‑70 (MQ=255)
                                                aCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcga                     <  1:816707/71‑3 (MQ=255)
                                                 ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa                    <  1:355606/71‑1 (MQ=255)
                                                    aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg                 >  1:604286/1‑71 (MQ=255)
                                                    aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg                 >  1:2983578/1‑71 (MQ=255)
                                                     cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc                <  1:1218457/71‑1 (MQ=255)
                                                      gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg               <  1:2561273/71‑1 (MQ=255)
                                                        atatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt             <  1:200674/71‑1 (MQ=255)
                                                        atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt             >  1:3215670/1‑71 (MQ=255)
                                                        atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt             >  1:98069/1‑71 (MQ=255)
                                                          atGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt             >  1:3049761/1‑69 (MQ=255)
                                                           tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa                                            >  1:3154818/1‑37 (MQ=255)
                                                             aTCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGtt        <  1:2785975/71‑1 (MQ=255)
                                                                ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt     <  1:3327877/71‑1 (MQ=255)
                                                                ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt     >  1:1556131/1‑71 (MQ=255)
                                                                  cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg   <  1:3675487/71‑1 (MQ=255)
                                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt  <  1:3690880/71‑1 (MQ=255)
                                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt  <  1:1734870/71‑1 (MQ=255)
                                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt  <  1:2664677/71‑1 (MQ=255)
                                                                    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg   >  1:2686193/1‑69 (MQ=255)
                                                                    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg   >  1:2177215/1‑69 (MQ=255)
                                                                     |                                                                    
CCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGT  >  minE/835076‑835213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: