Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,162 A→G 8.6% Y1207C (TAC→TGC) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,1620AG 8.6% 230.9 / 11.1 81Y1207C (TAC→TGC) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base G (5/2);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

ATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAA  >  minE/835092‑835231
                                                                      |                                                                     
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                                       >  1:429898/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                                       >  1:3549546/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                                       >  1:1251060/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa                                                                       >  1:2098608/1‑71 (MQ=255)
 tCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATAc                                                                      <  1:3102207/71‑1 (MQ=255)
   cATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACgg                                                                    <  1:3298958/71‑1 (MQ=255)
     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                                  <  1:1131923/71‑1 (MQ=255)
     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                                  >  1:3395370/1‑71 (MQ=255)
     tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                                  <  1:1992560/71‑1 (MQ=255)
      aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                                                 >  1:2771878/1‑71 (MQ=255)
      aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                                                 >  1:3048541/1‑71 (MQ=255)
       aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt                                                                 >  1:1526720/1‑70 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg                                                                     >  1:2633978/1‑62 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg                                                                     >  1:716266/1‑62 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                                             <  1:681022/70‑1 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                                             >  1:1438096/1‑70 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                                             >  1:1998371/1‑70 (MQ=255)
           ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac                                                             >  1:314025/1‑70 (MQ=255)
            gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta                                                           <  1:1524965/71‑1 (MQ=255)
            gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta                                                           <  1:566995/71‑1 (MQ=255)
             tCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAactac                                                          >  1:2711088/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACTGCTTTAActacta                                                        <  1:1967627/71‑1 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt                                                                <  1:1847513/63‑1 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt                                                                <  1:3653860/63‑1 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt                                                                  <  1:2014893/61‑1 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        >  1:3427113/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        >  1:2688957/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        >  1:1197218/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        >  1:3344492/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        >  1:491929/1‑71 (MQ=255)
               aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta                                                        <  1:992946/71‑1 (MQ=255)
                cccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTAt                                                       <  1:2930574/71‑1 (MQ=255)
                   gTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGc                                                    <  1:26367/71‑1 (MQ=255)
                        aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt                                               <  1:3088007/71‑1 (MQ=255)
                         cGCCGAAACGCACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa                                              <  1:3688406/71‑1 (MQ=255)
                         cGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa                                              <  1:2263758/71‑1 (MQ=255)
                          gCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAg                                             <  1:3084032/71‑1 (MQ=255)
                           ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg                                            >  1:3466709/1‑71 (MQ=255)
                           ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg                                            >  1:530452/1‑71 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                                      >  1:3650211/1‑60 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                                      >  1:2442846/1‑60 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                                      >  1:2168642/1‑60 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg                                                      >  1:909239/1‑60 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt                                           <  1:109088/71‑1 (MQ=255)
                            cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt                                           <  1:1185876/71‑1 (MQ=255)
                             gAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCgg                                            >  1:408821/1‑69 (MQ=255)
                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGGACCGTCggat                                          >  1:1525433/1‑68 (MQ=255)
                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGt                                               >  1:24556/1‑65 (MQ=255)
                              aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc                                         >  1:1549236/1‑71 (MQ=255)
                               aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc                                         >  1:2450242/1‑70 (MQ=255)
                                aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt                                        >  1:2220365/1‑70 (MQ=255)
                                aCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcga                                       <  1:816707/71‑3 (MQ=255)
                                 ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa                                      <  1:355606/71‑1 (MQ=255)
                                    aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg                                   >  1:2983578/1‑71 (MQ=255)
                                    aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg                                   >  1:604286/1‑71 (MQ=255)
                                     cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc                                  <  1:1218457/71‑1 (MQ=255)
                                      gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg                                 <  1:2561273/71‑1 (MQ=255)
                                        atatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt                               <  1:200674/71‑1 (MQ=255)
                                        atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt                               >  1:98069/1‑71 (MQ=255)
                                        atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt                               >  1:3215670/1‑71 (MQ=255)
                                          atGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt                               >  1:3049761/1‑69 (MQ=255)
                                           tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa                                                              >  1:3154818/1‑37 (MQ=255)
                                             aTCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGtt                          <  1:2785975/71‑1 (MQ=255)
                                                ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt                       >  1:1556131/1‑71 (MQ=255)
                                                ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt                       <  1:3327877/71‑1 (MQ=255)
                                                  cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                     <  1:3675487/71‑1 (MQ=255)
                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                    <  1:2664677/71‑1 (MQ=255)
                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                    <  1:3690880/71‑1 (MQ=255)
                                                   ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                    <  1:1734870/71‑1 (MQ=255)
                                                    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                     >  1:2686193/1‑69 (MQ=255)
                                                    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                     >  1:2177215/1‑69 (MQ=255)
                                                       aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg                >  1:727580/1‑71 (MQ=255)
                                                        tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg                              >  1:1602763/1‑56 (MQ=255)
                                                        tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                     <  1:3122389/65‑1 (MQ=255)
                                                            cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt             >  1:2342998/1‑69 (MQ=255)
                                                             aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt             >  1:3067849/1‑68 (MQ=255)
                                                                tGGCATACGGCTTTAACTACTATGGCACCGTAGGtt                                          >  1:131592/1‑36 (MQ=255)
                                                                tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAg          >  1:804932/1‑68 (MQ=255)
                                                                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga      <  1:3546/70‑1 (MQ=255)
                                                                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga      <  1:1801173/70‑1 (MQ=255)
                                                                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg       <  1:1718797/69‑1 (MQ=255)
                                                                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAAcc                                    <  1:1314845/37‑1 (MQ=255)
                                                                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa  >  1:2276106/1‑71 (MQ=255)
                                                                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa  >  1:3550476/1‑71 (MQ=255)
                                                                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag    <  1:1816253/69‑1 (MQ=255)
                                                                      |                                                                     
ATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAA  >  minE/835092‑835231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: