Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,931,909	(ATC)2→3	coding (615/999 nt)	idnR ←	DNA‑binding transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,931,902	1	.	C	100.0%	115.2 / NA	31	L208V (CTG→GTG)	idnR	DNA‑binding transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (31/0);  total (31/0)
*	minE	2,931,902	2	.	A	100.0%	96.6 / NA	31	L208L (CTG→TTG)	idnR	DNA‑binding transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (31/0);  total (31/0)
*	minE	2,931,902	3	.	T	100.0%	106.0 / NA	31	L208M (CTG→ATG)	idnR	DNA‑binding transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (31/0);  total (31/0)

CGTGGATTCATGCGTAATGGGGAAAGATTATGCAG‑‑‑CATCATCGCATCGCAATACCCCT  >  minE/2931868‑2931925                                    |||                        cgTGGATTCATGCGTAATGGGGAAAGATTATgcag‑‑‑ca                       >  1:2077764/1‑37 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:3106821/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2699868/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2737207/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2804073/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2819233/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2823413/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:299216/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2993012/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2694848/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:3310480/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:3362760/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:3563747/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:3644636/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:451008/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:484691/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:565376/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1337352/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2668439/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2491412/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2458086/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2437841/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:2094686/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:200039/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1954273/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:185123/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1781966/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1519692/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1474184/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1418669/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1397635/1‑61 (MQ=255) cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt  >  1:1357061/1‑61 (MQ=255)                                    |||                        CGTGGATTCATGCGTAATGGGGAAAGATTATGCAG‑‑‑CATCATCGCATCGCAATACCCCT  >  minE/2931868‑2931925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑