Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	minE	2164521	2164554	34	51 [0]	[0] 83	yrbF	predicted toluene transporter subunit

CTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGA  >  minE/2164460‑2164520                                                             | cTGCAACGCCTGGGCACTTCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2645776/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGATCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:751967/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3397338/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2644103/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:26581/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2666235/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:281147/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2994033/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3059492/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3071755/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3167067/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3189969/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3351684/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:961819/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3522361/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:427902/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:471430/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:47383/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:652441/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:689796/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:78551/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:791716/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:840995/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1050611/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2031812/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1059572/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1147620/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1312215/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1369807/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1374919/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1431997/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1474102/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1636174/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1783634/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:1798982/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2017196/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2066041/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2076332/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2082927/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2191054/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2323467/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2425738/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2436160/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2480039/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2501903/61‑1 (MQ=255) cTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2601797/61‑1 (MQ=255)  tGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:30768/60‑1 (MQ=255)   gCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3168300/59‑1 (MQ=255)                  tGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:3571839/44‑1 (MQ=255)                      aTGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:521029/40‑1 (MQ=255)                         aGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGa  <  1:2630172/37‑1 (MQ=255)                                                             | CTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTGTCCGCCAGGATCCAGGCGTGA  >  minE/2164460‑2164520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑