| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,049 | 0 | C | T | 41.7% | 83.2 / 151.1 | 120 | Y430Y (TAC→TAT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (70/0); new base T (0/50); total (70/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-35 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 120 total aligned reads displayed.
AGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCA > minE/2065001‑2065107 | ggCGGTTGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2560383/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2519057/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1962716/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3599425/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2213685/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2252727/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2256178/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3572035/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3534422/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:23306/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2342826/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2444982/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2467463/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3522409/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:907328/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2535117/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3473958/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3327492/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2741361/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3312722/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2768813/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2875146/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2875193/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3211259/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2932611/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3208731/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1060166/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1317036/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1299511/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1390847/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1168523/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1481656/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1157873/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:152487/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:668823/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1628038/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:590326/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:17835/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:589133/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1862745/53‑1 (MQ=255) cTACGCAACCTGGTTGATCCGTCAGGCGATCACCCGCTCTAt > 1:3531941/1‑42 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGCTCAGGCGCGCACCa > 1:3507203/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc > 1:1210462/1‑50 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCCCCa > 1:2939660/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:843431/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3161469/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:78992/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2693464/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3181639/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3177617/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:431438/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:337592/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3418957/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:74250/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:730990/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3507366/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:657627/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:630706/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:618521/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3578057/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:510619/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3594360/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:376632/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:427515/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:170214/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3109852/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2150912/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2044868/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2017622/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1938951/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1855412/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1816720/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1713081/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2181235/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1644734/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1605311/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1579589/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1444669/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1439675/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1020864/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1011266/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2281432/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3099088/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:3021587/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:300832/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2933503/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2928801/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2755944/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2698022/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:1007561/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2635871/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2623396/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2488432/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2473913/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:247227/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2310134/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2292368/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCa > 1:2267706/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCAGATCAGGCGCGCACCa > 1:614301/1‑62 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATAGCGGATCAGGCGCGCACCa > 1:1076315/1‑62 (MQ=255) | AGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCA > minE/2065001‑2065107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |