| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,068,600 | 0 | G | A | 33.3% | 89.0 / 70.6 | 75 | A397V (GCC→GTC) | aer | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base G (50/0); new base A (25/0); total (75/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTC > minE/2068552‑2068649 | gCATCAATCAGCTTGCGTATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:3300219/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:2774918/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:833413/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:77500/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:676103/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:447434/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:3630915/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:3167316/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:3121612/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:3117355/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:2763090/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:2333533/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1817597/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1756842/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1029324/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1562149/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1559298/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1242719/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaa > 1:1305392/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTa > 1:308433/1‑52 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTa > 1:543561/1‑52 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:2826944/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:1432529/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:2924048/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:1576901/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:136137/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:320530/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:335141/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:3431879/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:3502720/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:3571616/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:380489/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:411391/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:418489/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:573530/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:589008/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:1072236/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:2331010/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:2148547/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:1740849/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:154912/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:2403825/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTaa > 1:1659867/1‑53 (MQ=255)gCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGACTa > 1:1981319/1‑52 (MQ=255)gCATCAATCAGCTTGCGAATATCGTAGGCAGCATTAGCGCTGCGGCTGACTaa > 1:482471/1‑53 (MQ=39) gcgcTGCGGCTGGTTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1341899/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGccacca > 1:2241204/1‑40 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCGAAACCTTTGCCCTGTTc > 1:3143369/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt > 1:1591642/1‑61 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:816099/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1942023/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1934711/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1791014/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:548777/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:3449628/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1668811/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1181347/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:776130/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:703089/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2121200/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2164658/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1012771/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2171812/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:3383288/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2180816/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:3322382/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:3290433/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:3133882/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2379769/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2887887/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2842046/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:1518741/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCCTTGCCCTGTTc > 1:1364651/1‑62 (MQ=255) gcgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAAACTTTGCCCTGTTc > 1:188794/1‑62 (MQ=255) gagcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc > 1:2839588/3‑62 (MQ=255) | GCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTC > minE/2068552‑2068649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |