| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,741,879 | 0 | T | C | 30.3% | 151.5 / 94.7 | 109 | S30P (TCG→CCG) | rplV | 50S ribosomal subunit protein L22 |
| Reads supporting (aligned to +/- strand): ref base T (22/54); new base C (33/0); total (55/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 109 total aligned reads displayed.
GTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACCAACAA > minE/2741823‑2741913 | gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGCCCTGATTCGCGGTAAGAAAGTGCCggt > 1:673421/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2311128/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:3638589/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1673868/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1674753/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:3622431/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2362480/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:183258/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:3602679/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2392042/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:3310899/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:3035833/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:196243/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2257607/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2009980/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2210214/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2135987/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2774884/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:958024/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:265024/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:110290/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:898338/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1158870/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2473843/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:2760597/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:125269/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1292339/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1360308/1‑59 (MQ=255)gTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGCCggt > 1:1509279/1‑59 (MQ=255) aGGTTCGCCTTGTTGCTTACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2654458/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGGAAGTGTCGCAGGCTCTGGa < 1:1071727/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2958959/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3043896/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3047525/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2687513/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2872484/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3490212/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2927283/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:390390/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:98614/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:911911/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:91140/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:798761/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:790856/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:666780/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:576280/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:54709/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3122869/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3614186/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:106995/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3352413/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:328744/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3245368/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:3202887/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:312965/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1941400/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1056350/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1078233/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:112392/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1238390/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1331193/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1558855/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1590460/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1662342/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1763821/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1774678/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1839197/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2637234/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1951934/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2100410/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2578427/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2404575/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2273488/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2103136/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2131758/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2218988/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2504587/55‑1 (MQ=255) aGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:2208430/55‑1 (MQ=255) aGGTGCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:604184/55‑1 (MQ=255) cTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGa < 1:1072395/47‑1 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTg > 1:407410/1‑47 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1354735/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1242531/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:2788626/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1158806/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:981983/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:2996577/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:2329887/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3153669/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1670958/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3512396/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:187269/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1887944/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3264463/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:1986207/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3234384/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3131728/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACcaacaa > 1:3191010/1‑61 (MQ=255) cTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGAAATTTTGACCTACACcaacaa > 1:2523636/1‑61 (MQ=255) cTGACCTGAATCGCGGTAAGAAAGTGTCGCAGGCTCTCGATATTTTGACCTACACcaacaa > 1:2785709/1‑61 (MQ=255) | GTTCTTCTGCTCAGAAGGTTCGCCTTGTTGCTGACCTGATTCGCGGTAAGAAAGTGTCGCAGGCTCTGGATATTTTGACCTACACCAACAA > minE/2741823‑2741913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |