| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,240,909 | 1 | . | A | 61.7% | 195.8 / 144.2 | 94 | coding (342/870 nt) | yebK | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (23/13); new base A (0/58); total (23/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCAAAAAA > minE/1240868‑1240956 | tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:961243/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:2399082/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:2174750/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:2933493/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:3029869/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:3246232/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:3324995/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:3328949/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:1602971/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:362784/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:949193/61‑1 (MQ=255)tCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACcgcg < 1:1200789/61‑1 (MQ=255) cATGTCCGTCATTCACTGGTTT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1643030/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTTCTCACTCAgg < 1:337697/62‑1 (MQ=38) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:919223/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3004419/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2811056/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2799554/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2764102/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:275614/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2678417/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2559405/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2509080/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2489832/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2453801/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2401119/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:248401/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2369286/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2950077/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:492854/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:80907/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3105877/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:780513/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:648955/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3359120/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3402841/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3412687/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:637032/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3414900/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:567031/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:38339/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:478578/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1878695/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1517908/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1646351/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1728154/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1480299/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1775669/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1807941/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1809569/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1834286/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:147301/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1860246/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1376020/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1308048/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1987658/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2061494/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2078035/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1206808/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:113614/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2242401/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1112220/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2331676/62‑1 (MQ=255) cATGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCCCCGTCGACTTGCTCACTCAgg < 1:36838/62‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:1250572/61‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:557590/61‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:894158/61‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:3287561/61‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:2273656/61‑1 (MQ=255) aTGTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGGCGACTTGCTCACTCAgg < 1:2303493/61‑1 (MQ=255) gTCCGTCATTCACTGG‑ATAAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAgg < 1:103312/59‑1 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCg > 1:448412/1‑40 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:185545/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:2430861/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1157801/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:2168352/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:827537/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:817197/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1185509/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:2058286/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:161637/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1412757/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:46841/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1728406/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:381168/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:3390790/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1629831/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:3473670/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaaa > 1:1537268/1‑62 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaa > 1:183762/1‑61 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaa > 1:1896468/1‑61 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaa > 1:2768766/1‑61 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaaa > 1:2078478/1‑61 (MQ=255) cGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCaaaa > 1:389514/1‑60 (MQ=255) | TCCGCAATGGCAACGCTTGATCATGTCCGTCATTCACTGG‑AT‑AAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCAAAAAA > minE/1240868‑1240956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |