| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,594 | 0 | C | T | 74.6% | 249.0 / 100.8 | 138 | A283A (GCC→GCT) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (35/0); new base T (103/0); total (138/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 138 total aligned reads displayed.
CTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAAT > minE/1314541‑1314635 | cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATGTAAAGCCGAGTTta > 1:3295185/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGttt > 1:1255883/1‑60 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:1546545/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:3551422/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:175384/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:3307656/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:140077/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2009117/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:1357159/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:134520/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:592960/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:1284314/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:611835/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2091674/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2213325/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:3253506/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:1162836/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2348923/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:254784/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2671508/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2715839/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2892980/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:957486/1‑61 (MQ=255)cTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTta > 1:2942433/1‑61 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTTCCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:974078/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAgcg > 1:2871945/1‑38 (MQ=25) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGTCGATCTCAGCACCGAGAt > 1:2538187/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCCCCGaga > 1:3161950/1‑59 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCg > 1:1521688/1‑58 (MQ=39) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGaga > 1:2078311/1‑59 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGaga > 1:1960110/1‑59 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGaga > 1:1903512/1‑59 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:927464/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:750340/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:76659/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:800438/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3157839/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:836308/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2481915/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:934175/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3112294/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3059339/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2988948/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:948501/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2965991/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:395996/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3288457/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:691065/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:329362/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:618417/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:524077/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:508904/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:334166/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3366321/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3375658/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3468209/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3493689/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3524440/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:448387/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3555959/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:3565802/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:392160/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1200622/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1594130/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1527082/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:149991/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1450445/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1388390/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1312/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1280150/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1222289/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1206108/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1649151/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1182299/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1145081/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:114091/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1128959/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1124680/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1076734/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1054549/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1035357/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1922309/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2799826/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:278490/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2780917/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:103207/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2308821/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2224764/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2146541/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2054641/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:204537/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:2901698/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1866641/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1840884/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1822687/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1819638/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1767138/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1758433/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1724099/1‑62 (MQ=255) ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt > 1:1694834/1‑62 (MQ=255) | CTTGCCCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAAT > minE/1314541‑1314635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |