| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,370,325 | 0 | C | A | 50.0% | 23.2 / 71.5 | 50 | T93N (ACC→AAC) | yohK | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (22/3); new base A (0/25); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCAGCGTGGTTGCGATGGTGACGGGGACTTCCGTGGCATTGT > minE/1370276‑1370381 | ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1597524/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2696585/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:3341226/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2462466/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:3376342/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2190204/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:34967/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2040562/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1960934/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1918111/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1907798/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2819649/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1682961/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1564173/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:2847036/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1458974/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1339592/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:416433/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1297608/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1246372/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:614771/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:1186843/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:115663/62‑1 (MQ=255)ttATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:3198429/62‑1 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3088996/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3215698/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:2472920/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3288188/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:350272/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3617047/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3648383/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:66870/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:966747/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:1924435/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:1024324/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:1325434/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:1860650/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3077697/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:2182853/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:2233007/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:1007230/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:2797419/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:2812882/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCa > 1:3049545/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTCATCGGCa > 1:29120/1‑62 (MQ=255) tGAGCAGCTACACCAGATCCGCGCGCGCAGGAAATCGATCATCACCATCTGTTTTTTCGGCa > 1:390230/1‑62 (MQ=255) agcagcTACACCAGATCCGCGCGCGCTGGAAATCGATCATCAACATCTGTTTTAt < 1:751951/55‑1 (MQ=255) catcaCCATCTGTTTTATCGGCAGCGTGGTTGCGATGGTGACGGGGACTTCCGTGGCAttgt < 1:1501724/62‑1 (MQ=255) catcaCCATCTGTTTTATCGGCAGCGTGGTTGCGATGGTGACGGGGACTTCCGTGGCAttgt < 1:1240347/62‑1 (MQ=255) catcaCCATCTGTTTTATCGGCAGCGTGGTTGCGATGGTGACGGGGACTTCCGTGGCAttgt < 1:1096210/62‑1 (MQ=255) | TTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGCAGCGTGGTTGCGATGGTGACGGGGACTTCCGTGGCATTGT > minE/1370276‑1370381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |