Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,648,641	1	.	T	47.7%	138.8 / 199.2	86	intergenic (‑74/‑139)	yfgO/yfgC	predicted inner membrane protein/predicted peptidase
Reads supporting (aligned to +/- strand):  ref base . (45/0);  new base T (41/0);  total (86/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTAA  >  minE/1648623‑1648688                    |                                                gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAAc                          >  1:330876/1‑42 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTtgtgtg        >  1:3417216/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2442401/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2145274/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:406654/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3611208/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2206296/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2222194/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2310635/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2329350/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2403436/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2409503/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3468291/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:241979/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3458007/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2426276/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1015961/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2466303/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2577838/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2673725/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2742384/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2834714/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2845289/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3106541/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3117301/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3158341/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:3277510/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:533435/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:426424/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1848346/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2080889/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:2079662/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1237513/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:432880/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:543210/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1980901/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:711924/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1939368/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1591459/1‑60 (MQ=255) gTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:1922811/1‑60 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTgg       >  1:2318176/1‑58 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGc     >  1:1458598/1‑60 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:562429/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:3306022/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:1446630/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:1299637/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:328862/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:413393/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:1919591/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:2096519/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCt    >  1:2143112/1‑61 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:561163/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:560711/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:668450/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3371841/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3365539/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:480364/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3592319/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3608090/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:734060/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:98381/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3069951/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1189716/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1214767/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1241269/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1325590/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1575573/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1580550/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1659249/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1815652/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:1923830/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2034893/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2144024/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2156036/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2272772/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2337349/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2419032/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2423475/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2544392/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:2944015/1‑62 (MQ=255)     caAAAAAGACTTCGCTTTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTa   >  1:3177154/1‑62 (MQ=255)     caAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTg        >  1:15101/1‑56 (MQ=255)     caAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTaa  >  1:2101974/1‑62 (MQ=255)     caAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTaa  >  1:2161263/1‑62 (MQ=255)     caAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTaa  >  1:49357/1‑62 (MQ=255)     caAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTaa  >  1:3563909/1‑62 (MQ=255)                    |                                                GTCACAAAAAAGACTTCGC‑TTTTTATGACGGATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTAA  >  minE/1648623‑1648688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑