Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	26,319	G→A	A90T (GCT→ACT)	dapB →	dihydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	26,319	0	G	A	100.0%	77.0 / NA	24	A90T (GCT→ACT)	dapB	dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/0);  total (24/0)

GATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCC  >  minE/26271‑26331                                                 |             gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:275421/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:781958/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:57544/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:3633503/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:3223441/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:3072488/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:301767/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2999891/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2904372/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2872447/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1053059/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2553405/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2376569/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2356503/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2207842/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:2081379/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1605973/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1529694/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1408974/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1217591/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1109093/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGc   >  1:3137623/1‑60 (MQ=255) gATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCACTTTTAGTCGcc  >  1:3285988/1‑61 (MQ=255) gATGTGTTTATCGATTTTACCCGACCGGAAGGTACGCTGAACCATCTCACTTTTTGTCGcc  >  1:1178102/1‑61 (MQ=255)                                                 |             GATGTGTTTATCGATTTTACCCGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCC  >  minE/26271‑26331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑