Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,093,824	G→A	L851L (CTC→CTT)	ydiJ ←	predicted FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,093,824	0	G	A	100.0%	103.8 / NA	33	L851L (CTC→CTT)	ydiJ	predicted FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/2);  total (31/2)

GCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAA  >  minE/1093808‑1093872                 |                                                 gCTTCGCCATACGGTTAAGGAAATCCGCCGTCttttt                              >  1:1369413/1‑37 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGt                        >  1:3058515/1‑43 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:94734/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:105077/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:932874/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:812877/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:3623966/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:3476686/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2863776/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:281098/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2795287/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:27843/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2522580/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2436863/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2352719/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:2305139/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:1385019/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:1947525/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:1880497/1‑61 (MQ=255) gCTTCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAg      >  1:1915637/1‑61 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTc                       >  1:2778298/1‑42 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:2907490/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:3246621/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:336230/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:2869837/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:3571706/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:180647/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:411961/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:546960/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:1674170/1‑62 (MQ=255)   ttCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa   >  1:1536909/1‑62 (MQ=255)    tCGCCATACGGTTAAGGAAATCCGTCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaaa  <  1:1976510/62‑1 (MQ=255)    tCGCCATACGGTTAAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaaa  <  1:931039/62‑1 (MQ=255)                 |                                                 GCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAA  >  minE/1093808‑1093872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑