| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,126,914 | G→A | intergenic (‑281/+3) | pheS ← / ← pheM | phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,126,914 | 0 | G | A | 75.0% | 128.1 / 43.1 | 68 | intergenic (‑281/+3) | pheS/pheM | phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (17/0); new base A (0/51); total (17/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCAT > minE/1126873‑1126957 | ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGAt > 1:2600135/1‑44 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:2924896/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:3456529/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:2172466/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:2166712/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:358802/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:1985379/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:1958522/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:3040405/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:243616/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:2535235/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:270338/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:2799850/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:1494619/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:809170/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:870662/1‑61 (MQ=255)ggCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTaaa > 1:1231647/1‑61 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2992546/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3005764/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2991393/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2940894/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3221827/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3353399/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3406583/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:361186/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3558217/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2686746/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3613104/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:3631006/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:407804/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:423405/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:429257/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:471177/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:518199/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:690475/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:704024/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:717214/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:720491/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:871434/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1997444/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:121770/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1300069/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1344874/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1587408/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1606239/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1705154/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1778461/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1787549/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1821466/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1848532/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1891745/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:1921380/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2928756/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2025171/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2061156/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2142926/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2182988/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2215950/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2370296/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2419094/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2591188/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2638131/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:114083/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2898613/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCCGAAAATAGCAGcat < 1:3504801/60‑1 (MQ=255) cgcgCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:708838/59‑1 (MQ=255) cgcgCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:145306/59‑1 (MQ=255) gCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat < 1:2180033/52‑1 (MQ=38) | GGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCAT > minE/1126873‑1126957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |