Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,126,935	A→G	F9F (TTT→TTC)	pheM ←	phenylalanyl‑tRNA synthetase operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,126,935	0	A	G	100.0%	187.4 / NA	51	F9F (TTT→TTC)	pheM	phenylalanyl‑tRNA synthetase operon leader peptide
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/51);  total (0/51)

ACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCAT  >  minE/1126898‑1126957                                      |                       aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3613104/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:114083/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2898613/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2928756/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2940894/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2991393/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2992546/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3005764/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3221827/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3353399/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3406583/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3558217/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:361186/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2686746/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:3631006/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:407804/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:423405/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:429257/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:471177/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:518199/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:690475/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:704024/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:717214/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:720491/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:871434/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1891745/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:121770/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1300069/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1344874/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1587408/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1606239/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1705154/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1778461/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1787549/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1821466/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1848532/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2638131/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1921380/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:1997444/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2025171/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2061156/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2142926/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2182988/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2215950/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2370296/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2419094/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2591188/60‑1 (MQ=255) aCGCGCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCCGAAAATAGCAGcat  <  1:3504801/60‑1 (MQ=255)  cgcgCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:145306/59‑1 (MQ=255)  cgcgCTAGCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:708838/59‑1 (MQ=255)         gCCTCCTGAAGTCAGGTGCTAAAGTAAAAGAAGAAGCGGAAAATAGCAGcat  <  1:2180033/52‑1 (MQ=38)                                      |                       ACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCAT  >  minE/1126898‑1126957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑