Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,138,492	A→G	*197* (TAA→TAG)	ydjM →	predicted inner membrane protein regulated by LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,138,492	0	A	G	100.0%	49.9 / NA	14	*197* (TAA→TAG)	ydjM	predicted inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/14);  total (0/14)

CAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCA  >  minE/1138438‑1138499                                                       |        cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:103011/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:1459692/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:172152/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:2126159/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:2371407/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:2700802/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:3013470/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:331686/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:543873/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:716389/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:771272/62‑1 (MQ=255) cAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:81904/62‑1 (MQ=255)  aaTACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:1170336/61‑1 (MQ=255)              aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAGAAAGGCa  <  1:1254745/49‑1 (MQ=255)                                                       |        CAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCA  >  minE/1138438‑1138499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑