Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,225,029	T→C	I297I (ATT→ATC)	yebU →	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,225,029	0	T	C	100.0%	233.3 / NA	60	I297I (ATT→ATC)	yebU	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (60/0);  total (60/0)

GTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTG  >  minE/1224977‑1225037                                                     |         gTGCAAACAAAGTGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3014112/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAAtata        >  1:2445477/1‑55 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACt   >  1:2729464/1‑60 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACt   >  1:1706895/1‑60 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACt   >  1:3047882/1‑60 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3495596/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3513400/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3471413/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3357098/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3184958/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3081927/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3021205/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3016122/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3012264/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2924000/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:275114/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2739156/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:955990/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3520994/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:356144/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:3645541/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:41848/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:508632/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:635382/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:738023/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:764734/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:765195/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:810948/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:839188/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:85667/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:941861/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:102386/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1738638/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1050013/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1086675/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1204008/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1207456/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1219922/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1228469/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1324319/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1472866/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1521577/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1547942/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1559624/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1651023/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1707462/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1727474/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2570746/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1751514/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:176632/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1793403/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1922916/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1934762/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:194191/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:1973178/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2059731/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2119576/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2248491/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2260968/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2284829/1‑61 (MQ=255) gTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATCTACGACTg  >  1:2445313/1‑61 (MQ=255)                                                     |         GTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTG  >  minE/1224977‑1225037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑