Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,769,780	T→C	intergenic (+211/‑60)	yfiO → / → yfiA	predicted lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,769,780	0	T	C	100.0%	98.4 / NA	27	intergenic (+211/‑60)	yfiO/yfiA	predicted lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/27);  total (0/27)

AAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACATCA  >  minE/1769729‑1769790                                                    |           aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1308284/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:450725/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3632621/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3391248/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3353510/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3350922/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:334545/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3125904/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3107942/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3094674/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:3044948/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2685160/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2561932/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2450004/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2406378/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2293197/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2213701/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2205972/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:2135356/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1761389/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1751491/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1577917/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1469919/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1369609/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1318457/62‑1 (MQ=255) aaaaaGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:1220867/62‑1 (MQ=255)     aGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACACTTTGACATCa  <  1:995566/58‑1 (MQ=255)                                                    |           AAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACATCA  >  minE/1769729‑1769790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑