Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,815,306	A→G	*167R (TGA→CGA)	recX ←	regulatory protein for RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,815,306	0	A	G	100.0%	74.5 / NA	21	*167R (TGA→CGA)	recX	regulatory protein for RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/0);  total (21/0)

AAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGC  >  minE/1815262‑1815322                                             |                 aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCTGCAAAATTTCGc  >  1:3275234/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2732276/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:884352/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:765681/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:723685/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:3506676/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:3449062/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:3291346/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2948902/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2898280/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2805158/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:1137106/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2284823/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2282588/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2248913/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2157114/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:2078514/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:1995493/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:16882/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:1531805/1‑61 (MQ=255) aagataagTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCGGTCGGCAAAATTTCGc  >  1:1376307/1‑61 (MQ=255)                                             |                 AAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGC  >  minE/1815262‑1815322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑