Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,961,085 C→T D122N (GAC→AAC)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,961,0850CT100.0% 490.0 / NA 139D122N (GAC→AAC) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (83/56);  total (83/56)

Only 100 of 166 total aligned reads displayed.

GAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTCGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTC  >  minE/1961026‑1961121
                                                           |                                    
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:1902945/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:340791/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:443481/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:3274331/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:3153316/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:2643094/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:2272228/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:665259/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:681925/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:309607/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:729000/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:836461/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:3005595/62‑1 (MQ=255)
gAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTATTGGACAGGGTTTcgttgt                                    <  1:259375/62‑1 (MQ=255)
gAATGAGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:136361/62‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:909884/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:2087596/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:1807954/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:3388911/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:2987261/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:2112439/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:2194447/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:1577914/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:1237371/59‑1 (MQ=255)
             aCGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:1210567/59‑1 (MQ=255)
                      tCACACAGTGCAGCGTATTTTTTGGACAGGGTTTcgttgt                                    <  1:451175/40‑1 (MQ=255)
                             gTGCAGCGTATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:289307/43‑1 (MQ=255)
                                     tATTTTTTGGACAGGGTTTCGTTGTCTTTCTtctc                          <  1:1319196/35‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:2727375/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1052804/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:2541941/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:302602/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:3554108/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:3639460/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:377959/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:497114/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:55679/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:641172/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:775974/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:87485/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1728367/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1964582/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1169046/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:187975/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1534538/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1071005/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:1591846/50‑1 (MQ=255)
                                       ttttttGGACAGGGTTTCGTTCTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:2891304/50‑1 (MQ=255)
                                        tttttGGACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGaa         <  1:766973/49‑1 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:346165/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:406312/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:1744644/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:463921/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:508333/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:3123189/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:3045548/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:3605331/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2017692/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2470643/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2477972/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:951682/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2558788/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:1021351/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2232412/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTttgttg                >  1:2770402/1‑36 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAATGTTTTc  >  1:2861670/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGtttt   >  1:1501448/1‑49 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGtttt   >  1:2027786/1‑49 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:788463/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1109705/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1505953/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1131405/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1450838/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:410220/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1356100/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1310809/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2399450/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:128123/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:993061/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:305753/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2511480/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2364210/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:230506/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2620560/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2650721/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2230547/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2838367/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2849661/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:2112567/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:294542/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:3593274/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1953145/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:310350/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1825488/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:3278384/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1796521/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:340372/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:1745946/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:3527385/1‑50 (MQ=255)
                                              gACAGGGTTTCGTTGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTc  >  1:3533894/1‑50 (MQ=255)
                                                           |                                    
GAATGCGTCCATTACGAACACGTCACACAGTGCAGCGTATTTTTTGGACAGGGTTTCGTCGTCTTTCTTCTCGCCTTTGTTGAAGCGAACGTTTTC  >  minE/1961026‑1961121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑