Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,962,687	T→C	intergenic (‑170/+115)	epd ← / ← yggC	D‑erythrose 4‑phosphate dehydrogenase/conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,962,687	0	T	C	100.0%	186.6 / NA	49	intergenic (‑170/+115)	epd/yggC	D‑erythrose 4‑phosphate dehydrogenase/conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

TCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAT  >  minE/1962636‑1962708                                                    |                      tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:2557524/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:943350/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:646040/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:581678/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:576462/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:497587/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:3386459/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:3250965/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:3127377/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:2956645/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:2584694/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:2424440/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:2317637/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:1945477/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:1898228/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:1893949/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:1472458/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:172540/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:1359184/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:127326/1‑61 (MQ=255) tCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGc              >  1:17454/1‑61 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGTAGACAt  <  1:3339838/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3623195/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3371855/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3567833/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3150161/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1325330/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1214009/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1202115/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:660689/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:808231/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:924945/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1089580/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3197600/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1586520/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:3107146/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2959224/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2744640/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2616936/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1059851/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1749594/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2264151/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:225005/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2164615/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2082925/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:2066445/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:1849909/62‑1 (MQ=255)            gCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGCTTGGCCAGCCGAAGAAGACAt  <  1:1745401/62‑1 (MQ=255)                                   gTGGAATAAAAGGAATGCTTGTCCAGCCGAAGAAGACAt  <  1:826239/39‑1 (MQ=255)                                                    |                      TCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAT  >  minE/1962636‑1962708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑