| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,005,397 | T→C | D118G (GAC→GGC) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,005,397 | 0 | T | C | 100.0% | 21.7 / NA | 7 | D118G (GAC→GGC) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/0); total (7/0) | |||||||||||
AAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGT > minE/2005389‑2005442 | aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:1466238/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:2271090/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:2303494/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:3110193/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:3365208/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:440168/1‑54 (MQ=255)aaGTGATGCCCACCCAGTCAAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:1814614/1‑54 (MQ=255) | AAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGT > minE/2005389‑2005442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |