| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,065,007 | T→G | V416V (GTT→GTG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,007 | 0 | T | G | 98.0% | 181.0 / NA | 50 | V416V (GTT→GTG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (0/49); total (0/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAC > minE/2065001‑2065054 | ggCGGTTGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2560383/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3327492/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2741361/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2768813/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:283795/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2875146/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2875193/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2932611/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2936566/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3124998/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3208731/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3211259/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3312722/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:932242/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3473958/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3522409/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3534422/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3572035/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:3599425/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:419979/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:589133/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:590326/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:668823/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:706929/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:907328/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1060166/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1862745/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1101495/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1157873/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1168523/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1299511/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1317036/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1390847/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1481656/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:152487/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:156165/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1628038/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:17835/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2535117/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:1962716/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2156296/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2213685/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2252727/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2256178/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:23306/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2342826/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2429880/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2444982/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2467463/53‑1 (MQ=255)ggCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTATGCGAc < 1:2519057/53‑1 (MQ=255) | AGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAC > minE/2065001‑2065054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |