Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,151,535	G→A	A165V (GCT→GTT)	ftsH ←	protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,151,535	0	G	A	100.0%	275.9 / NA	86	A165V (GCT→GTT)	ftsH	protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (86/0);  total (86/0)

GCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGA  >  minE/2151486‑2151589                                                  |                                                       gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2760886/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2665577/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2595120/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2926192/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2322346/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2289725/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2198081/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:2024682/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:3464423/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:467638/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:3643928/1‑62 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:2753635/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:2889471/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:2321992/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:232080/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:2012100/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:1409275/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:741696/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:599908/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:903670/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttct                                             >  1:540031/1‑61 (MQ=255) gccgAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttc                                              >  1:2069999/1‑60 (MQ=255)  gccaGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAACAActtcttctt                                            >  1:443809/4‑61 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCa                               >  1:1104455/1‑39 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGcc                            >  1:3502637/1‑42 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTc         >  1:3095616/1‑61 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:2877866/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:934576/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1119488/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:758878/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:2919059/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:393440/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:297264/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:646037/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:622986/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:3326352/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:3404416/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:578103/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:3437514/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:540481/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:498089/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:3541942/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:3577070/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:269884/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1624788/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1618339/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1678085/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1733654/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1760730/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1796955/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1828055/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1839625/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1881598/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1571470/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1434899/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1418167/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:2296611/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1332391/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1320299/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:2581875/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:2623089/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:1157246/1‑62 (MQ=255)                                     cTCAACCAGTTCAACAACCTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCg        >  1:511912/1‑62 (MQ=255)                                     cTCAACCAGTTAAACAACTTCTTCTTTTGCATCGTCGCAGCCCGCac                       >  1:2310400/1‑46 (MQ=37)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:1418863/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:70768/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:1543255/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:849782/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:869701/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:1303589/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:934625/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:496002/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:167771/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2193403/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2250062/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:3398663/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:3384334/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:3339818/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:3172770/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2382422/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2437230/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2925920/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2677607/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2814375/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTtga  >  1:2774083/1‑62 (MQ=255)                                           cAGTTCAACAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGGTTtga  >  1:3118724/1‑62 (MQ=255)                                                  |                                                       GCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGA  >  minE/2151486‑2151589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑